Histochemical study of long nuclear chain fibers in the cat muscle spindle
Histochemical study of long nuclear chain fibers in the cat muscle spindle
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猫肌梭长核链纤维的组织化学研究
DOI：
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发表时间：
1980
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通讯作者：
J. Kučera
中科院分区：
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作者：
J. Kučera
用组织化学方法对猫梭内肌纤维在肌梭的连续横断面上进行了观察。用“肌原纤维”三磷酸腺苷酶染色反应识别309个纺锤体的核囊和核链纤维。40根核链纤维在纺锤体被膜的末端延伸1,000μm或更长。与典型的短极链状纤维相比，这些长链纤维对烟酰胺腺嘌呤二核苷酸四唑还原酶(NADH-TR)的染色强度较低。在胆碱酯酶(ChE)染色的切片上，大多数长链纤维的包膜外区域可见一到两个短而致密的“板状”ChE沉积，这可能代表骨骼梭形轴突的终末。此外，约三分之一的长链纤维显示一个或多个较细和较小的胆碱酯酶活动区域，可能对应于梭形运动轴突的末梢。典型链状纤维的整体ChE染色模式不同于长链状纤维。然而，一些较短的核链纤维类似于具有NADH-TR反应的长链纤维，尽管它们的ChE“板”位于包膜内。
Cat intrafusal muscle fibers were examined histochemically in serial transverse sections of tenuissimus muscle spindles. The “myofibrillar” adenosine triphosphatase staining reaction was used to recognize the nuclear bag and the nuclear chain fibers in 309 spindle poles. Poles of 40 nuclear chain fibers extended for 1,000 μm or more beyond the termination of the spindle capsule. These long chain fibers stained less intensely for nicotinamide adenine dinucleotide tetrazolium reductase (NADH‐TR) than the typical chain fibers of shorter polar length. In sections stained for cholinesterases (ChE), the extracapsular regions of most long chain fibers displayed one or two short, dense “plate”‐type ChE deposits, which may represent the terminals of skeleto‐fusimotor axons. In addition, about one‐third of the long chain fibers displayed one or more thinner and smaller areas of ChE activity, possibly corresponding to the endings of fusimotor axons. The overall ChE staining pattern of the typical chain fibers was unlike that of the long chains. However, some of the shorter nuclear chain fibers resembled long chain fibers with the NADH‐TR reaction, even though their ChE “plates” were located intracapsularly.