A flexizyme that selectively charges amino acids activated by a water-friendly leaving group
A flexizyme that selectively charges amino acids activated by a water-friendly leaving group
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DOI：
10.1016/j.bmcl.2009.03.114
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发表时间：
2009-07-15
影响因子：
2.7
通讯作者：
Suga, Hiroaki
中科院分区：
文献类型：
--
作者：
Niwa, Nobuyoshi;Yamagishi, Yusuke;Suga, Hiroaki
我们开发了一种新的柔酶（一种柔性从头tRNA酰化核酶）系统，一对氨基衍生的苯甲基硫酯（ABT）和氨基柔酶（aFx）。ABT轴承铵离子的设计，使酰基供体基板更好的水溶性。虽然以前报道的flexizyme（eFx和dFx）没有显示出对ABT衍生物的酰化活性，但通过体外选择氨基酸活化的ABT产生的新的flexizyme变体aFx对那些活化的ABT表现出高选择性。包括aFx、eFx和dFx的flexizymes系统使我们能够制备各种各样的带有非蛋白质氨基酸的酰基-tRNA。(C)2009爱思唯尔有限公司保留所有权利。
We have developed a new flexizyme (a flexible de novo tRNA acylation ribozyme) system, a pair of amino-derivatized benzyl thioester (ABT) and amino flexizyme (aFx). ABT bearing the ammonium ion was designed to render the acyl-donor substrates better water solubility. Although the previously reported flexizymes (eFx and dFx) did not show acylation activity for the ABT derivatives, a new flexizyme variant aFx, generated by in vitro selection against an amino acid activated ABT, exhibits high selectivity toward those activated ABT. The flexizymes system including aFx, eFx, and dFx enables us to prepare a wide variety of acyl-tRNAs charged with non-proteinogenic amino acids. (C) 2009 Elsevier Ltd. All rights reserved.