Effects of Introduction of Sorghum RbcS with Rice RbcS Knockdown by RNAi on Photosynthetic Activity and Dry Weight in Rice.
Effects of Introduction of Sorghum RbcS with Rice RbcS Knockdown by RNAi on Photosynthetic Activity and Dry Weight in Rice.
复制标题
引入高粱 RbcS 并通过 RNAi 敲除水稻 RbcS 对水稻光合活性和干重的影响。
DOI：
10.1080/1343943x.2020.1847669
复制
发表时间：
2021
影响因子：
2.5
通讯作者：
Hiroyoshi Matsumura & Hiroshi Fukayama
中科院分区：
文献类型：
--
作者：
Kazuma Sakoda ;Akito Yamamoto ;Chie Ishikawa ;Yojiro Taniguchi ;Hiroyoshi Matsumura & Hiroshi Fukayama
Rubisco是光合作用固定CO2的关键酶。在此之前，我们通过在水稻中过量表达高粱C4型Rubisco小亚基（RbcS）成功地提高了Rubisco的催化速率（kcat）。这些Rubisco是高粱RbcS和水稻RbcS的嵌合体。在本研究中，我们将水稻RbcS的RNAi构建体导入高粱RbcS过表达系（SS 10）。获得的双转基因株系iSS 4和iSS 5主要表达高粱RbcS，积累的Rubisco含量分别为非转基因水稻（WT）的35%和20%。在这些双转基因株系中，Rubisco对CO2的kcat和Km均高于WT和SS10。此外，iSS 4在高CO2条件下表现出较高的光合氮利用效率。这些结果表明C4型RbcS在提高CO2浓度条件下的光合性能方面是有用的。
Rubisco is the key enzyme responsible for photosynthetic CO2fixation. Previously, we succeeded to increase the catalytic rate (kcat) of Rubisco by the overexpression of sorghum C4-type Rubisco small subunit (RbcS) in rice. These Rubisco are present as a chimera of sorghum RbcS and rice RbcS. In this study, we introduced an RNAi construct for riceRbcSinto a sorghum RbcS overexpression line (SS10). Obtained double-transgenic lines iSS4 and iSS5 dominantly expressed sorghum RbcS and accumulated 35% and 20% of Rubisco content of non-transgenic rice (WT), respectively. Rubisco in these double-transgenic lines showed higherkcatandKmfor CO2than WT and SS10. Moreover, iSS4 showed higher photosynthetic nitrogen use efficiency under high CO2conditions. These results demonstrate the usefulness of C4-type RbcS to improve photosynthetic performance under elevated CO2conditions.