Feline immunodeficiency virus is shed in semen from experimentally and naturally infected cats.
Feline immunodeficiency virus is shed in semen from experimentally and naturally infected cats.
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猫免疫缺陷病毒通过实验和自然感染的猫的精液排出。
DOI：
10.1089/aid.1998.14.1087
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Tompkins,WA
中科院分区：
文献类型：
--
作者：
Jordan,HL;Howard,J;Barr,MC;Kennedy-Stoskopf,S;Levy,JK;Tompkins,WA
虽然实验室分离的猫免疫缺陷病毒（FIV），FIV-NCSU 1，已通过人工授精在家猫中传播，自然感染的男性在交配期间的传播尚未报告。为了确定精液中的病毒脱落是否是NCSU 1分离株所独有的，我们分析了来自四名感染另一种实验室菌株FIVPetaluma的无特定病原体男性和八名自然获得性感染的随机来源男性的电射精。通过巢式聚合酶链反应扩增法筛选感染Petaluma分离株的猫的精细胞裂解物中的FIV gagDNA。通过与猫CD 4 +T细胞系和Crandell猫肾细胞共培养，进一步分析这些FIV-Petaluma猫的精细胞和精浆中是否存在病毒。将自然感染猫的电射精与T细胞系共培养。我们的研究结果表明，无细胞的FIV存在于精浆从两个FIV-Petaluma猫和两个自然感染的猫。在所有的FIV-Petaluma感染的猫和一个自然感染的猫中检测到细胞相关的精液病毒。分泌的病毒gag p26抗原，一个积极的病毒复制的迹象，是显而易见的，在共培养物中含有游动精子纯化的游泳程序从FIV-Petaluma猫。这些结果证实，FIV脱落在精液中不限于特定的病毒分离。此外，来自感染FIV的猫的上游精子在体外可能具有感染性。
Although a laboratory isolate of feline immunodeficiency virus (FIV), FIV-NCSU1, has been transmitted by artifical insemination in domestic cats, transmission by naturally infected males during mating has not been reported. In order to determine whether virus shedding in semen is unique to the NCSU1isolate, we analyzed electroejaculates from four specific-pathogen-free males infected with another laboratory strain, FIVPetaluma, and eight random source males with naturally acquired infections. Seminal cell lysates from the cats infected with the Petaluma isolate were screened by nested polymerase chain reaction amplification for FIVgagDNA. Seminal cells and seminal plasma from these FIV-Petaluma cats were further analyzed for the presence of virus by cocultivation with a feline CD4+T cell line and Crandell feline kidney cells. Electroejaculates from the naturally infected cats were cocultivated with the T cell line. Our results demonstrated that cell-free FIV was present in seminal plasma from two FI V-Petaluma cats and two naturally infected cats. Cell-associated seminal virus was detected in all of the FIV-Petaluma infected cats and one naturally infected cat. Secretion of viral gag p26 antigen, an indication of active viral replication, was evident in cocultures containing motile sperm purified by a swim-up procedure from a FIV-Petaluma cat. These results confirm that FIV shedding in semen is not restricted to a specific virus isolate. Furthermore, swim-up sperm from FIV-in-fected cats may be infectiousin vitro.