DISEASE DEVELOPMENT IN ETHYLENE-INSENSITIVE ARABIDOPSIS-THALIANA INFECTED WITH VIRULENT AND AVIRULENT PSEUDOMONAS AND XANTHOMONAS PATHOGENS
DISEASE DEVELOPMENT IN ETHYLENE-INSENSITIVE ARABIDOPSIS-THALIANA INFECTED WITH VIRULENT AND AVIRULENT PSEUDOMONAS AND XANTHOMONAS PATHOGENS
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DOI：
10.1094/mpmi-5-372
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发表时间：
1992-09-01
影响因子：
3.5
通讯作者：
STASKAWICZ, BJ
中科院分区：
文献类型：
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作者：
BENT, AF;INNES, RW;STASKAWICZ, BJ
植物激素乙烯已被假设在疾病抗性和疾病易感性中发挥作用。这些过程是通过使用等基因的有毒和无害的拟南芥细菌病原体和突变体来检测的，这些突变体改变了乙烯生理。拟南芥乙烯不敏感ein1和ein2突变体对丁香假单胞菌具有抗性。添加克隆的无毒基因avrRpt2、avrRpm1或avrB使番茄无毒；这表明乙烯并不需要对无毒细菌产生积极的抗性。在第二组实验中，用毒性p.s.v v监测敏感性。番茄，p.s.p v。黄斑单胞菌，或油菜黄单胞菌pv。定压力。野生型拟南芥和ein1突变体对这些菌株敏感，但ein2突变体仅产生最小的疾病症状。尽管症状有所减轻，但毒性p.s. pv。番茄在叶片内广泛生长。假单胞菌植物毒素coronatine在许多植物物种上诱导乙烯生物合成和疾病样症状，但ein2突变体的症状减轻不能归因于对coronatine不敏感。ein2植物的耐受性增强表明乙烯可能介导了病原体诱导的损伤，但ein1突变体缺乏耐受性尚未得到解释。
The plant hormone ethylene has been hypothesized to play roles both in disease resistance and in disease susceptibility. These processes were examined by using isogenic virulent and avirulent bacterial pathogens and mutants of Arabidopsis thaliana that were altered in ethylene physiology. Ethylene-insensitive ein1 and ein2 mutants of Arabidopsis were resistant to Pseudomonas syringae pv. tomato made avirulent by the addition of the cloned avirulence genes avrRpt2, avrRpm1, or avrB; this suggests that ethylene is not required for active resistance against avirulent bacteria. In a second set of experiments, susceptibility was monitored with virulent P. s. pv. tomato, P. s. pv. maculicola, or Xanthomonas campestris pv. campestris strains. Wild-type Arabidopsis and ein1 mutants were susceptible to these strains, but ein2 mutants developed only minimal disease symptoms. Despite these reduced symptoms, virulent P. s. pv. tomato grew extensively within ein2 leaves. The Pseudomonas phytotoxin coronatine induces ethylene biosynthesis and diseaselike symptoms on many plant species, but the reduced symptomology of ein2 mutants could not be attributed to insensitivity to coronatine. The enhanced disease tolerance of ein2 plants suggests that ethylene may mediate pathogen-induced damage, but the absence of tolerance in ein1 mutants has yet to be explained.