Decreased expression of Beclin 1 in eutopic endometrium of women with adenomyosis
Decreased expression of Beclin 1 in eutopic endometrium of women with adenomyosis
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DOI：
10.1007/s00404-009-1280-0
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发表时间：
2010-10-01
影响因子：
2.6
通讯作者：
Zheng, Wei
中科院分区：
文献类型：
--
作者：
Ren, Yuefang;Mu, Lin;Zheng, Wei
研究人员已经启动了自噬相关基因Beclin 1的发热研究的新领域。我们的目的是探讨Beclin 1的表达是否改变在位子宫内膜的妇女子宫腺肌病及其与临床feature.Methods我们收集了组织样本，从在位子宫内膜的30名妇女子宫腺肌病和32名健康妇女接受手术的良性指征。我们培养的基质细胞的在位神经元。结果Beclin 1 mRNA在子宫腺肌症患者在位内膜间质细胞和子宫内膜组织中的表达均明显低于正常对照组（P <0.0 5）; Beclin 1蛋白在异位子宫内膜间质细胞和子宫腺肌病子宫内膜组织中的表达也明显低于对照组（P < 0.01）。Beclin 1蛋白表达与血清CA 125（r =-0.307，P = 0.015）、盆腔疼痛（r =-0.542，P = 0.000）呈负相关。Beclin 1与血清CA 125、盆腔疼痛呈负相关。Beclin 1可能参与了子宫内膜异位症的发生和发展。对子宫腺肌病的自噬作用还需进一步研究。
Purpose Researchers have launched a new area of febrile investigations on the autophagy-related gene Beclin 1. Our aim is to investigate whether Beclin 1 expression is altered in eutopic endometrium of women with adenomyosis and its association with clinical characteristics.Methods We collected tissue samples from the eutopic endometria of 30 women with adenomyosis and 32 healthy women undergoing surgery for benign indications. We cultured the stromal cells of the eutopic endometria. Beclin I expression of the cultured stromal cells and tissues was assessed by reverse transcription polymerase chain reaction and western blot analysis.Results Beclin 1 messenger RNA (mRNA) expression in cultured stromal cells of eutopic endometria and endometrial tissues of women with adenomyosis was significantly lower than that of controls (P < 0.05). Beclin 1 protein expression in cultured stromal cells of eutopic endometria and endometrial tissues of adenomyosis was also significantly lower compared with that of controls (P < 0.01). Beclin 1 protein expression in eutopic endometrial tissues was negatively correlated with serum CA 125 (r = -0.307, P = 0.015), and pelvic pain (r = -0.542, P = 0.000).Conclusions The study revealed Beclin I mRNA and protein expression were significantly decreased in eutopic endometria of women with adenomyosis. Moreover, Beclin 1 was negatively correlated with serum CA125 and pelvic pain. Beclin 1 might contribute to the pathogenesis and progression of endometriosis. Further research on autophagy of adenomyosis is required.