Rapid Access to Giant Unilamellar Liposomes with Upper Size Control: Membrane-Gated, Gel-Assisted Lipid Hydration
Rapid Access to Giant Unilamellar Liposomes with Upper Size Control: Membrane-Gated, Gel-Assisted Lipid Hydration
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DOI：
10.1021/acs.langmuir.0c01496
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发表时间：
2020-11-10
期刊：
影响因子：
3.9
通讯作者：
Zhan, Wei
中科院分区：
文献类型：
--
作者：
Liu, Zening;Cui, Jinyan;Zhan, Wei
结合凝胶辅助脂质水合和基于膜的脂质挤出，我们在这里展示了快速制备具有上部尺寸控制的巨型单层脂质体的一般程序。在这个过程中的特点是平面脂质堆积沉积在聚乙烯醇凝胶上，其上进一步层压微孔聚碳酸酯膜。因此，脂质体的大小控制是通过后者的均匀大小的孔隙来实现的，这些孔隙为底层脂质在水化后进入主水相提供了唯一的通道。采用几种常用的模型脂质，包括1,2-二棕榈酰基- cn -甘油-3-磷酸胆碱（DPPC）、1,2-二油基- cn -甘油-3-磷酸胆碱（DOPC）、1-棕榈酰基-2-油基-甘油-3-磷酸胆碱（POPC）和胆固醇，生产单相和双相（Janus）脂质体。用共聚焦荧光显微镜对这些脂质体的大小分布、产率和层状结构进行了详细的表征和分析。因此，该程序提供了一个简单而快速的替代途径，以较大的尺寸控制巨大的单层脂质体。
Combining gel-assisted lipid hydration with membrane-based lipid extrusion, we demonstrate here a general procedure for rapid preparation of giant unilamellar liposomes with upper size control. Featured in this procedure are planar lipid stacks deposited on poly(vinyl alcohol) gel, which are further laminated atop with microporous polycarbonate membranes. Control of liposome size is thus realized through the uniform-sized pores of the latter, which provide the only access for the underlying lipids to enter the main aqueous phase upon hydration. Production of both single-phased and biphasic (Janus) liposomes using several commonly employed model lipids, including 1,2-dipalmitoyl-sn-glycero3-phosphocholine (DPPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) and cholesterol, is presented. The size distribution, yield and lamellarity of these liposome products are characterized and analyzed in detail by confocal fluorescence microscopy. This procedure thus offers a simple and fast alternative route to giant unilamellar liposomes with upper size control.