Region-specific and epileptogenic-dependent expression of six subtypes of α2,3-sialyltransferase in the adult mouse brain
Region-specific and epileptogenic-dependent expression of six subtypes of α2,3-sialyltransferase in the adult mouse brain
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DOI：
10.1046/j.1471-4159.2003.01257.x
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发表时间：
2003-01-01
影响因子：
4.7
通讯作者：
Kato, K
中科院分区：
文献类型：
--
作者：
Matsuhashi, H;Horii, Y;Kato, K
唾液酸糖结合物在多种生物学功能中发挥着重要作用。在大脑中也观察到了这些结构，并提出唾液酸化可能会影响神经的可塑性。为了阐明唾液酸化对大脑的影响，应该首先确定表现唾液酸化的特定神经元。应用原位杂交技术，系统研究了生理刺激和非生理刺激条件下编码6种α-2，3-唾液酸基转移酶(ST3GalI-VI)的mRNAs在成年小鼠脑内的定位。首先，观察到了显著的区域特异性表达模式：ST3Gal II、III和V mRNAs位于整个大脑的神经细胞中，而ST3Gal I、IV和VI mRNAs位于有限的脑区。接下来，为了评估这六个mRNAs的表达是否可以被调节，我们检测了点燃致痫对这六个mRNAs水平的影响。在6种亚型中，丘脑ST3Gal IV表达水平上调最为明显，丘脑前核内表达ST3Gal IV的神经元数量在致痫过程中呈时间依赖性地从2%增加到21%。Western印迹分析检测丘脑终末产物的增加。这些发现为阐明唾液酸化糖共轭何时何地发挥作用提供了分子基础，并伴随着神经可塑性。
Sialylated glycoconjugates play important roles in various biological functions. The structures are also observed in brains and it has been proposed that sialylation may affect neural plasticity. To clarify the effects of sialylation in the brain, particular neurons that exhibit sialylation should first be determined. Using in situ hybridization, we performed systematic surveys of the localization of mRNAs encoding the six alpha2,3-sialyltransferases (ST3Gal I-VI) in the adult mouse brain with or without physiological stimulation. First, striking region-specific patterns of expression were observed: While ST3Gal II, III, and V mRNAs were in neuronal cells throughout the brain, ST3Gal I, IV, and VI mRNAs were in restricted brain regions. Next, to assess whether the expression of the six mRNAs can be regulated, we examined the effect of kindling epileptogenesis on the six mRNA levels. Of the six subtypes, upregulation in the ST3Gal IV level in the thalamus was most pronounced; the number of ST3Gal IV-expressing neurons in the anterior thalamic nuclei increased from 2% to 21% in a time-dependent manner during epileptogenesis. Western blot analysis evaluated the increase of the end-products in the thalamus. These findings provide a molecular basis to clarify when and where sialylated glycoconjugates function accompanied by neural plasticity.