Bent DNA is found in some, but not all, regions recognized by the Bacillus subtilis AbrB protein.
Bent DNA is found in some, but not all, regions recognized by the Bacillus subtilis AbrB protein.
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在枯草芽孢杆菌 AbrB 蛋白识别的部分（但不是全部）区域中发现了弯曲 DNA。
DOI：
10.1007/bf00290723
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Ayazifar,M
中科院分区：
文献类型：
--
作者：
Strauch,MA;Ayazifar,M
枯草芽孢杆菌的ABRB蛋白是多种代谢途径中众多基因的转录调节因子，在稳定期开始表达。AbrB是一种DNA结合蛋白，与其靶基因的特定DNA区域具有特定的亲和力。对这些区域的分析未能检测到AbrB结合的明显序列决定因素，人们认为AbrB识别由有限的碱基序列子集形成的特定DNA结构。我们检查了六个AbrB结合区域的固有弯曲，发现其中三个不包含可检测到的固有弯曲，而三个包含可检测到的固有弯曲。ABRB与这些区域的结合导致了电泳迁移率模式，这与DNA部分以弯曲构型存在是一致的，但没有证明。
The AbrB protein ofBacillus subtilisis a transcriptional regulator of numerous genes in diverse metabolic pathways that commence expression at the onset of stationary phase. AbrB is a DNA-binding protein with specific affinity for defined DNA regions of its target genes. Analysis of these regions has failed to detect a readily apparent sequence determinant for AbrB binding and it is believed that AbrB recognizes a specific DNA structure formed by a finite subset of base sequences. We have examined six AbrB binding regions for intrinsic bending and found that three do not contain a detectable intrinsic bend, whereas three do. AbrB binding to these regions resulted in electrophoretic mobility patterns that were consistent with, but did not prove, that the DNA moieties were present in a bent configuration.