Melatonin biosynthesis restored by CpG oligodeoxynucleotides attenuates allergic airway inflammation via regulating NLRP3 inflammasome.
Melatonin biosynthesis restored by CpG oligodeoxynucleotides attenuates allergic airway inflammation via regulating NLRP3 inflammasome.
复制标题
CpG 寡脱氧核苷酸恢复褪黑素生物合成，通过调节 NLRP3 炎性体减轻过敏性气道炎症。
DOI：
10.1016/j.lfs.2019.117067
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发表时间：
2019-11
期刊：
影响因子：
6.1
通讯作者：
Fei Guang-He
中科院分区：
文献类型：
--
作者：
Wu Hui-Mei;Xie Qiu-Meng;Zhao Cui-Cui;Xu Juan;Fan Xiao-Yun;Fei Guang-He
AimsBoth CpG oligodeoxynucleotide (CpG-ODN) and melatonin have been reported to induce Th1 response and contribute to allergic asthma resistance. Here, we aimed to reveal how they confer such effect as well as whether they crosstalk with each other.Main methodsSix-week-old Female C57BL/6 mice were challenged by OVA to induce allergic airway inflammation, and were treated with CpG-ODN, CpG-ODN plus Luzindole or melatonin respectively. Bronchoalveolar lavage fluid (BALF) cellularity was classified and counted by Wright's-Giemsa staining. HE and PAS staining were used to analyze airway inflammation. The levels of IL-4, IL-5, IL-13,GM-CSF and IFN-γ, as well as IL-1β and IL-18 were analyzed by ELISA. Protein expressions of ASMT, AANAT, NLRP3, IL-1β and caspase-1 in lung tissue were detected by Western blotting, expression of ASMT and AANAT were further observed by immunohistochemistry.Key findingsWe found that CpG-ODN considerably suppressed OVA-induced airway leukocytes infiltration, goblet cell hyperplasia and Th2 cytokines production. Furthermore, the resolution effect of CpG-ODN on OVA-induced allergic airway inflammation occurred in parallel with decreased-activation of NLRP3 inflammasome and increased biosynthesis of melatonin. Blocking the effect of endogenous melatonin by Luzindole abolished the suppressive effect of CpG-ODN on OVA-induced airway inflammation and activation of NLRP3 inflammasome, suggesting such effect was mediated by endogenous melatonin. Moreover, exogenous melatonin pronouncedly ameliorated airway inflammation and decreased the activation of NLRP3 inflammasome.SignificanceThese results proven that CpG-ODN protects against allergic airway inflammation via suppressing the activation of NLRP3 inflammasome, and such effect may be resulted from the restored-production of melatonin.
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影响因子：
6.1
作者：
García-Mauriño, S;Pozo, D;Guerrero, JM
通讯作者：
Guerrero, JM
影响因子：
4.4
作者：
Li, Hanfen;Willingham, Stephen B.;Ting, Jenny P. -Y.;Re, Fabio
通讯作者：
Re, Fabio
影响因子：
10.3
作者：
Hui-Mei Wu;Q. Shen;L. Fang;Shi-Hai Zhang;Peiming Shen;Ya-Jing Liu;Rong-Yu Liu
通讯作者：
Hui-Mei Wu;Q. Shen;L. Fang;Shi-Hai Zhang;Peiming Shen;Ya-Jing Liu;Rong-Yu Liu
影响因子：
4.4
作者：
J. Kline;T. Waldschmidt;T. Businga;J. E. Lemish;J. Weinstock;P. Thorne;A. Krieg
通讯作者：
J. Kline;T. Waldschmidt;T. Businga;J. E. Lemish;J. Weinstock;P. Thorne;A. Krieg
影响因子：
14.2
作者：
Jain, VV;Kitagaki, K;Kline, JN
通讯作者：
Kline, JN