A technique of isolation and perfusion of the canine liver
A technique of isolation and perfusion of the canine liver
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DOI：
10.1016/s0022-4804(61)80023-1
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发表时间：
1961-01-01
期刊：
影响因子：
--
通讯作者：
McDERMOTT, WILLIAM V.
中科院分区：
文献类型：
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作者：
KESTENS, PAUL J.;FARRELLY, JULIA A.;McDERMOTT, WILLIAM V.
使用分离的灌注动物肝脏进行这些实验的最初目的是获得可再现的实验制备物，其将作为正常肝脏发挥功能。关于流量、氧利用率和肝功能参数的报告结果表明，这一点已经实现。由于最初遇到的困难，特别是在狗身上，我们和其他工作人员，似乎值得详细报告这项技术。“流出道阻塞”和灌注不满意的原因太多，无法详细描述。从体内肝脏到离体灌注制备的快速过渡和Meaning技术是必不可少的。即使是短暂的缺氧也会增加门静脉床阻力，并且仅通过门静脉灌注高氧饱和度的血液将不能建立令人满意的灌注。正常的pCO2是防止动脉床阻力增加和进行性缺氧所必需的，pH必须保持在7.2至7.5之间。目前正在详细研究缺氧、酸碱平衡改变和其他代谢变化对流速和肝功能的影响。|| 摘要编辑：作者
The initial objective of these experiments with the isolated, perfused animal liver was to achieve a reproducible experimental preparation that would function as a normal liver. The results reported as to flow, oxygen utilization and parameters of liver function indicate that this has been accomplished. Because of the initial difficulties encountered, particularly in the dog, by us and by other workers, it seemed worth while to report the technique in detail. Causes of "outflow block" and unsatisfactory perfusions are too numerous to describe in detail. Meticulous technique and a smooth rapid transition from the in vivo liver to the isolated perfused preparation are essential. Even brief periods of anoxia increase the portal bed resistance and perfusion through the portal vein alone of blood of high oxygen saturation will not establish a satisfactory perfusion. A normal pCO2 is necessary to prevent increasing resistance in the arterial bed and progressive hypoxia, and pH must be maintained between 7.2 and 7.5. Detailed studies on the effects of hypoxia, alterations in acid-base balance and other metabolic changes on flow rates and liver function are in progress. || ABSTRACT AUTHORS: Authors