Mannose-binding lectin is produced by vaginal epithelial cells and its level in the vaginal fluid is influenced by progesterone
Mannose-binding lectin is produced by vaginal epithelial cells and its level in the vaginal fluid is influenced by progesterone
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DOI：
10.1016/j.molimm.2010.07.016
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发表时间：
2010-11-01
影响因子：
3.6
通讯作者：
Tedesco, F.
中科院分区：
文献类型：
--
作者：
Bulla, R.;De Seta, F.;Tedesco, F.
甘露糖结合凝集素（MBL）是补体（C）系统的一种识别分子，与多种病原菌、病毒、真菌和寄生虫上的糖配体结合，在宫颈-阴道腔中检测到MBL，它可以作为抵抗生殖系统下生殖道感染性病原体的第一线防御。从11名月经周期不同阶段的正常骑自行车妇女中获得的结果显示，分泌期MBL水平增加。该MBL的一部分来自循环，如CVL中存在转铁蛋白所示，该转铁蛋白作为血管和组织渗透性的标志物。子宫内膜的作用可以忽略不计，因为子宫切除术RT-PCR和原位RT-PCR前后MBL水平没有变化。PCR分析表明，阴道组织，特别是上皮的基底层是MBL的来源，MBL与基底膜和上皮的外层细胞结合。表明在CVL中检测到的MBL来源于血浆（由于渗出）和局部合成，其水平在月经周期的分泌期呈孕酮依赖性增加（C）2010 Elsevier Ltd版权所有
Mannose-binding lectin (MBL) is a recognition molecule of the complement (C) system and binds to carbohydrate ligands present on a wide range of pathogenic bacteria viruses fungi and parasitesMBL has been detected in the cervico-vaginal cavity where it can provide a first-line defence against infectious agents colonizing the lower tract of the reproductive systemAnalysis of the cervico-vaginal lavage (CVL) obtained from 11 normal cycling women at different phases of the menstrual cycle revealed increased levels of MBL in the secretive phase Part of this MBL derives from the circulation as indicated by the presence of transferrin in CVL tested as a marker of vascular and tissue permeability The local synthesis of MBL is suggested by the finding that its level is substantially higher than that of transferrin in the secretive phase The contribution of endometrium is negligible since the MBL level did not change before and after hysterectomy RT-PCR and in situ RT-PCR analysis showed that the vaginal tissue and in particular the basal layer of the epithelium is a source of MBL which binds to the basal membrane and to cells of the outer layers of the epitheliumIn conclusion we have shown that MBL detected in CVL derives both from plasma as result of transudation and from local synthesis and its level is progesterone dependent increasing in the secretive phase of the menstrual cycle (C) 2010 Elsevier Ltd All rights reserved