Ganglionic blockade with tetraethylammonium in conscious rats.
Ganglionic blockade with tetraethylammonium in conscious rats.
复制标题
用四乙铵阻断清醒大鼠的神经节。
DOI：
10.1161/01.hyp.19.6.814
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
DiBona,GF
中科院分区：
文献类型：
--
作者：
Petersen,JS;DiBona,GF
交感神经节阻断剂四乙铵已被用作测量肾血浆流量的清除标记物，但大鼠中的交感神经节阻断剂量尚不清楚。鉴于交感神经活动对心脏和肾脏的反射激活差异，我们比较了四乙铵对肾神经活动、平均动脉压和心率的影响。清醒的大鼠被注射媒介物（等渗盐水）或四乙铵（两组中n = 7）。四乙铵按以下剂量累积输注（每剂35分钟）：每小时10(-5)、10(-4)、10(-3)、10(-2)和10(-1)g/kg体重。平均动脉压降低15%的剂量为10(-1)，心率为0.55×10(-1)，肾神经活动为每小时0.055×10(-1)g/kg体重。肾神经活性在每小时10(-1)g/kg体重时被消除；当剂量低于每小时10(-1)g/kg体重时，平均动脉压和心率没有变化。致死剂量为每小时1克/公斤体重。在媒介物处理的动物中没有观察到变化。每小时10(-1) g/kg体重的四乙铵导致乙酰胆碱诱导的平均动脉压降低期间肾神经活动的增加减弱，反映了动脉压力感受器抑制。我们得出结论，肾神经活动对交感神经节阻滞的敏感性分别比心率和平均动脉压高 10 倍和 18 倍。当四乙铵用作测量大鼠肾血浆流量的清除标志物时，每小时的给药剂量应小于10(-2) g/kg体重。
The sympathetic ganglionic blocking agent tetraethylammonium has been used as a clearance marker for the measurement of renal plasma flow, but the sympathetic ganglionic blocking dose in rats is unknown. In light of differential reflex activation of sympathetic nerve activity to heart and kidney, we compared the effect of tetraethylammonium on renal nerve activity, mean arterial pressure, and heart rate. Conscious rats were infused with either vehicle (isotonic saline) or tetraethylammonium (n = 7 in both groups). Tetraethylammonium was infused cumulatively (35 minutes per dose) in the following doses: 10(-5), 10(-4), 10(-3), 10(-2), and 10(-1) g/kg body wt per hour. Doses for 15% reduction were 10(-1) for mean arterial pressure, 0.55 x 10(-1) for heart rate, and 0.055 x 10(-1) g/kg body wt per hour for renal nerve activity. Renal nerve activity was abolished at 10(-1) g/kg body wt per hour; mean arterial pressure and heart rate were unchanged at doses lower than 10(-1) g/kg body wt per hour. The lethal dose was 1 g/kg body wt per hour. No changes were observed in vehicle-treated animals. Tetraethylammonium at 10(-1) g/kg body wt per hour resulted in an attenuated increase in renal nerve activity during acetylcholine-induced reduction in mean arterial pressure, reflecting arterial baroreceptor inhibition. We conclude that renal nerve activity is 10- and 18-fold more sensitive to sympathetic ganglionic blockade than heart rate and mean arterial pressure, respectively. When tetraethylammonium is used as a clearance marker for measurement of renal plasma flow in rats, it should be administered in doses less than 10(-2) g/kg body wt per hour.