Role of Bim in diallyl trisulfide-induced cytotoxicity in human cancer cells.
Role of Bim in diallyl trisulfide-induced cytotoxicity in human cancer cells.
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DOI：
10.1002/jcb.22896
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发表时间：
2011-01
影响因子：
4
通讯作者：
Lee, Yong J.
中科院分区：
文献类型：
--
作者：
Lee, Byeong-Chel;Park, Bae-Hang;Kim, Seog-Young;Lee, Yong J.
The aim of this study was to investigate the effect of garlic constituent diallyl trisulfide (DATS) on the cell death signaling pathway in a human breast cell line (MDA-MB-231). We observed that DATS (10–100 μM) treatment resulted in a dose- and time-dependent cytotoxicity. Treatment of MDA-MB-231 cells with a cytotoxicity inducing concentration of DATS (50–80 μM) resulted in an increase in the intracellular level of reactive oxygen species (ROS). Data from assay with MitoSOX™ Red reagent suggest that mitochondria are the main source of ROS generation during DATS treatment. DATS-induced oxidative stress was detected through glutaredoxin (GRX), a redox-sensing molecule, and subsequently GRX was dissociated from apoptosis signal-regulating kinase 1 (ASK1). Dissociation of GRX from ASK1 resulted in the activation of ASK1. ASK1 activated a downstream signal transduction JNK (C-Jun N-terminal kinase)-Bim pathway. SP600125, a JNK inhibitor, inhibited DATS-induced Bim phosphorylation and protected cells from DATS-induced cytotoxicity. Our results indicate that the cytotoxicity caused by DATS is mediated by the generation of ROS and subsequent activation of the ASK1-JNK-Bim signal transduction pathway in human breast carcinoma MDA-MB-231 cells.
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影响因子：
37.3
作者：
Shankar S;Ganapathy S;Chen Q;Srivastava RK
通讯作者：
Srivastava RK
影响因子：
6
作者：
O'Reilly, LA;Cullen, L;Strasser, A
通讯作者：
Strasser, A
影响因子：
11.2
作者：
Antosiewicz, Jedrzej;Herman-Antosiewicz, Anna;Singh, Shivendra V.
通讯作者：
Singh, Shivendra V.
影响因子：
7.8
作者：
Song, Jae J;Lee, Yong J
通讯作者：
Lee, Yong J
DOI：
10.1158/1078-0432.ccr-09-0512
发表时间：
2009-08-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Stan SD;Singh SV
通讯作者：
Singh SV