Application of microstereolithography in the development of three-dimensional cartilage regeneration scaffolds
Application of microstereolithography in the development of three-dimensional cartilage regeneration scaffolds
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DOI：
10.1007/s10544-007-9129-4
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发表时间：
2008-04-01
影响因子：
2.8
通讯作者：
Cho, Dong-Woo
中科院分区：
文献类型：
--
作者：
Lee, Seung-Jae;Kang, Hyun-Wook;Cho, Dong-Woo
制造三维（3-D）组织工程支架的传统方法具有很大的局限性。在本文中，我们提出了一种应用微立体光刻技术构建 3D 软骨支架的方法。该系统能够通过堆叠光聚合材料来制造具有预先设计的内部结构（例如孔径和孔隙率）的支架。为了控制支架结构，使用 CAD/CAM 技术生成支架图案算法。由于组织支架必须使用生物相容性、可生物降解的材料构建，因此使用液体光固化TMC/TMP合成支架，然后在末端进行丙烯酸化，并在紫外光照射下光固化。还评估了 TMC/TMP 聚合物的固化性能。为了评估支架功能，将软骨细胞接种在两种类型的 3-D 支架上并表征细胞粘附。结果表明支架几何形状在软骨细胞粘附中起着关键作用，最终影响支架的组织再生效用。这些 3D 支架最终可能会产生优化设计的结构，用于软骨和骨骼等各种组织的再生。
Conventional methods for fabricating three-dimensional (3-D) tissue engineering scaffolds have substantial limitations. In this paper, we present a method for applying microstereolithography in the construction of 3-D cartilage scaffolds. The system provides the ability to fabricate scaffolds having a pre-designed internal structure, such as pore size and porosity, by stacking photopolymerized materials. To control scaffold structure, CAD/CAM technology was used to generate a scaffold pattern algorithm. Since tissue scaffolds must be constructed using a biocompatible, biodegradable material, scaffolds were synthesized using liquid photocurable TMC/TMP, followed by acrylation at the terminal ends, and photocured under UV light irradiation. The solidification properties of the TMC/TMP polymer were also assessed. To assess scaffold functionality, chondrocytes were seeded on two types of 3-D scaffold and characterized for cell adhesion. Results indicate that scaffold geometry plays a critical role in chondrocyte adhesion, ultimately affecting the tissue regeneration utility of the scaffolds. These 3-D scaffolds could eventually lead to optimally designed constructs for the regeneration of various tissues, such as cartilage and bone.