Cell Systems to Investigate the Impact of Polyphenols on Cardiovascular Health.
Cell Systems to Investigate the Impact of Polyphenols on Cardiovascular Health.
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DOI：
10.3390/nu7115462
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发表时间：
2015-11-11
期刊：
影响因子：
5.9
通讯作者：
Van Camp J
中科院分区：
文献类型：
--
作者：
Grootaert C;Kamiloglu S;Capanoglu E;Van Camp J
多酚是一类植物来源的微量营养素，可用作抗氧化剂，对人类健康大体上有贡献。更具体地说，许多研究小组在几项动物研究和人体试验中调查了它们对心血管疾病的保护作用。然而，由于人类细胞和驻留的肠道微生物群落对多酚结构的过度处理，导致试验对象之间的差异很大，其保护作用的确切机制仍在调查中。为此，简化的细胞培养系统被用来减少机械学研究中的个体间变异性。在这篇综述中，我们将讨论迄今为止在心血管疾病背景下用于多酚研究的不同细胞培养模型。我们还将回顾细胞培养研究的当前趋势，包括共培养方法学。最后，我们将讨论这些高级模型的潜力，以筛选食品及其代谢物中存在的大量生物活性多酚对心血管的影响。
Polyphenols are a diverse group of micronutrients from plant origin that may serve as antioxidants and that contribute to human health in general. More specifically, many research groups have investigated their protective effect against cardiovascular diseases in several animal studies and human trials. Yet, because of the excessive processing of the polyphenol structure by human cells and the residing intestinal microbial community, which results in a large variability between the test subjects, the exact mechanisms of their protective effects are still under investigation. To this end, simplified cell culture systems have been used to decrease the inter-individual variability in mechanistic studies. In this review, we will discuss the different cell culture models that have been used so far for polyphenol research in the context of cardiovascular diseases. We will also review the current trends in cell culture research, including co-culture methodologies. Finally, we will discuss the potential of these advanced models to screen for cardiovascular effects of the large pool of bioactive polyphenols present in foods and their metabolites.