Physiochemical properties of various polymer substrates and their effects on microchip electrophoresis performance
Physiochemical properties of various polymer substrates and their effects on microchip electrophoresis performance
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DOI：
10.1016/j.chroma.2005.08.083
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发表时间：
2006-04-14
影响因子：
4.1
通讯作者：
Soper, SA
中科院分区：
文献类型：
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作者：
Shadpour, H;Musyimi, H;Soper, SA
一套聚合物进行了评估，其适用性作为可行的基板材料的微芯片电泳应用，这是通过复制技术制造。研究的相关理化性质包括玻璃化转变温度（T-g），紫外-可见吸收特性，自发荧光水平，电渗流（EVOF）和疏水性/亲水性，通过固着水接触角测量确定。这些物理化学性质被用作选择用于预期微芯片电泳应用的适当基底材料的指南。这些聚合物的Tg为优化压印参数以最小化复制误差（RE）提供了指导，该复制误差从表面轮廓仪迹线进行评估。对于聚合物聚碳酸酯（PC）和低密度聚乙烯（LDPE），RE值分别为0.4 - 13.6%。还在几个不同的波长下测量了聚合物的吸收光谱和自发荧光水平。在光学透明度方面（低吸收损失和小的自发荧光水平），聚（甲基丙烯酸甲酯）、PMMA（透明丙烯酸）提供了理想的特性，在488-780 nm范围内的激发波长下具有与玻璃相当的自发荧光水平。接触角测量显示最大值（即，高度疏水性），平均接触角为104度+/-3度，灰色丙烯酸G-PMMA表现出最小值，平均接触角为27度+/-2度。还测量了热组装芯片在用牛血清白蛋白（BSA）处理之前和之后的Δ ε。在四种不同的聚合物微芯片上，使用632.8 nm处的激光诱导荧光（LIF）激发，对染料标记的蛋白质（包括碳酸酐酶、磷酸化酶B、β-半乳糖苷酶和肌球蛋白）的混合物进行电泳分离。发现几个峰对的最大平均分辨率为5.04，使用BSA处理的PETG微芯片获得的肌球蛋白的效率为6.68 × 10（4）个板。(c)2005 Elsevier B. V.保留所有权利。
A suite of polymers were evaluated for their suitability as viable substrate materials for microchip electrophoresis applications, which were fabricated via replication technology. The relevant physiochemical properties investigated included the glass transition temperature (T-g), UV-vis absorption properties, autofluorescence levels, electroosmotic flow (EOF) and hydrophobicity/hydrophilicity as determined by sessile water contact angle measurements. These physiochemical properties were used as a guide to select the proper substrate material for the intended microchip electrophoretic application. The T-g of these polymers provided a guide for optimizing embossing parameters to minimize replication errors (REs), which were evaluated from surface profilometer traces. RE values ranged from 0.4 to 13.6% for the polymers polycarbonate (PC) and low-density polyethylene (LDPE), respectively. The absorption spectra and autofluorescence levels of the polymers were also measured at several different wavelengths. In terms of optical clarity (low absorption losses and small autofluorescence levels), poly(methyl methacrylate), PMMA (clear acrylic), provided ideal characteristics with autofluorescence levels comparable to glass at excitation wavelengths that ranged from 488-780 nm. Contact angle measurements showed a maximum (i.e., high degree of hydrophobicity) for polypropylene (PP), with an average contact angle of 104 degrees +/- 3 degrees and a minimum exhibited by gray acrylic, G-PMMA, with an average contact angle of 27 degrees +/- 2 degrees. The EOF was also measured for thermally assembled chips both before and after treatment with bovine serum albumin (BSA). The electrophoretic separation of a mixture of dye-labeled proteins including; carbonic anhydrase, phosphorylase B, beta-galactosidase, and myosin, was performed on four different polymer microchips using laser-induced fluorescence (LIF) excitation at 632.8 nm. A maximum average resolution of 5.04 for several peak pairs was found with an efficiency of 6.68 x 10(4) plates for myosin obtained using a BSA-treated PETG microchip. (c) 2005 Elsevier B.V. All rights reserved.