Oxidative stress as a prerequisite for aflatoxin production by Aspergillus parasiticus
Oxidative stress as a prerequisite for aflatoxin production by Aspergillus parasiticus
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DOI：
10.1016/s0891-5849(00)00398-1
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发表时间：
2000-11-15
影响因子：
7.4
通讯作者：
Subramanyam, C
中科院分区：
文献类型：
--
作者：
Jayashree, T;Subramanyam, C
通过比较产毒曲霉寄生菌株和非产毒曲霉寄生菌株在生长早期（营养期）和后期对数生长阶段（特发性）的需氧量和抗氧化状态，研究了自由基生成和氧化应激与黄曲霉毒素产生的相关性。与非产毒株相比，产毒株在各个生长阶段的需氧量相对不变，而产毒株在黄曲霉毒素产生的营养期表现出更大的需氧量。黄嘌呤氧化酶、超氧化物歧化酶和谷胱甘肽过氧化物酶等抗氧化酶的活性以及硫代巴比托酸活性物质和还原性谷胱甘肽的菌丝含量在产毒菌株从营养期向特发性生长的过程中均有所提高。综合结果表明，产毒菌株产生黄曲霉毒素可能是氧化应激增加导致脂质过氧化和自由基生成增强的结果。(C) 2000 Elsevier Science Inc.；
The relevance of free radical generation and oxidative stress with regard to aflatoxin production was examined by comparing the oxygen requirement and antioxidant status of a toxigenic strain of Aspergillus parasiticus with that of a nontoxigenic strain at early (trophophase) and late logarithmic (idiophase) growth phases. In comparison to the nontoxigenic strain, wherein the oxygen requirements were relatively unaltered at various growth phases, the toxigenic strain exhibited greater oxygen requirements at trophophase coinciding with onset of aflatoxin production. The activities of antioxidant enzymes such as xanthine oxidase, superoxide dismutase, and glutathione peroxidase and the mycelial contents of thiobarbituric acid-reactive substances as well as of reduced glutathione were all enhanced during the progression of toxigenic strain from trophophase to idiophase. The combined results suggest that aflatoxin production by the toxigenic strain may be a consequence of increased oxidative stress leading to enhanced lipid peroxidation and free radical generation. (C) 2000 Elsevier Science Inc.