Novel diazoresin/carboxymethyl chitosan capillary coating for the analysis of proteins by capillary electrophoresis
Novel diazoresin/carboxymethyl chitosan capillary coating for the analysis of proteins by capillary electrophoresis
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DOI：
10.1080/00150193.2018.1448184
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发表时间：
2018-06
期刊：
影响因子：
0.8
通讯作者：
Liping Zhou;Yao Chen;Hao Hu;Bing Yu;Guowei Wang;H. Cong
中科院分区：
文献类型：
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作者：
Liping Zhou;Yao Chen;Hao Hu;Bing Yu;Guowei Wang;H. Cong
毛细管电泳已成为分离和分析生物大分子的有效工具。本文提出了一种制备共价连接毛细管电泳涂料的新方法。以光敏重氮树脂（DR）为偶联剂，羧甲基壳聚糖为涂层。通过单层（LBL）自组装和DR独特的光化学反应，可以得到稳定高效的毛细管涂层。共价结合膜层具有抑制蛋白质在硅胶毛细管内表面吸附的能力，可实现溶菌酶（Lys）、肌红蛋白（Mb）、牛血清白蛋白（BSA）和核糖核酸酶a （RNase a）的基线分离。共价连接DR/羧甲基壳聚糖毛细管涂料具有良好的稳定性和重复性。该方法是一种环保、简便的制备毛细管电泳用共价膜的方法。
ABSTRACT Capillary electrophoresis has become an effective tool for the separation and analysis of biomolecule macromolecule.In this work, a new method to prepare covalent connection capillary electrophoretic coatings is proposed. Photosensitive diazoresin (DR) as coupling agents and carboxymethyl chitosan as coating was used. Through layer by layer (LBL) self-assembly and unique photochemistry reaction of DR, a stable and efficient capillary coating can be obtained. The covalently bonded coatings have the ability of suppressing protein adsorption on the inner surface of silica capillary, and thus a baseline separation of Lysozyme (Lys), myoglobin (Mb), bovine serum albumin (BSA) and ribonuclease A (RNase A) can be achieved. The covalently linked DR/carboxymethyl chitosan capillary coatings presented good stability and repeatability. This method seems to be an environmental and simple way to prepare the covalently coated capillaries for CE.