Genetic screens using the piggyBac transposon
Genetic screens using the piggyBac transposon
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DOI：
10.1016/j.ymeth.2010.12.022
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发表时间：
2011-04-01
期刊：
影响因子：
4.8
通讯作者：
Liu, Pentao
中科院分区：
文献类型：
--
作者：
Chew, Su Kit;Rad, Roland;Liu, Pentao
转座子是用于遗传筛选的有吸引力的系统，因为它们在分子上是易处理的，并且产生所需表型的破坏的基因座容易被定位。我们在此考虑piggyBac转座子系统的特征，以补充现有的哺乳动物筛选策略，包括睡美人转座子系统。我们还描述了我们为正向和反向遗传筛选开发的piggyBac资源的设计，以及我们在这些实验中使用的协议。(C)2010年爱思唯尔公司All rights reserved.
Transposons are an attractive system to use in genetic screens as they are molecularly tractable and the disrupted loci that give rise to the desired phenotype are easily mapped. We consider herein the characteristics of the piggyBac transposon system in complementing existing mammalian screen strategies, including the Sleeping Beauty transposon system. We also describe the design of the piggyBac resources that we have developed for both forward and reverse genetic screens, and the protocols we use in these experiments. (C) 2010 Elsevier Inc. All rights reserved.