Fast Characterization of Polyplexes by Taylor Dispersion Analysis
Fast Characterization of Polyplexes by Taylor Dispersion Analysis
复制标题
通过泰勒色散分析快速表征聚合体
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
H. Cottet
中科院分区：
文献类型：
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作者：
L. Leclercq;Sören Reinhard;Joseph Chamieh;M. Döblinger;E. Wagner;H. Cottet
在单一程序中，泰勒弥散分析(TDA)用于表征多聚体的大小，并对多聚体样品中过量的自由聚阳离子进行定量。TDA分析在额部模式下进行，以提高检测的灵敏度。概念的验证是使用线性聚赖氨酸(DP 20)和来自鲑鱼睾丸的DNA在氮磷比(N/P)为12时产生的模型复合体来建立的。将复合体的流体动力学半径与动态光散射测量值进行了比较。TDA被发现提供了权平均流体动力学半径，而DLS基本上给出了强度平均值(调和z平均值)。然后，该方法被应用于从不同DP(50,100)和不同拓扑结构(第2代和第3代树枝接枝多赖氨酸)的聚赖氨酸中发出的各种聚合物的研究。这一新的方法学将极大地促进用于玻璃纤维的聚合物的物理化学表征。
In a single procedure, Taylor dispersion analysis (TDA) was used for the size characterization of polyplexes and the quantification of free polycation contained in excess within the polyplex sample. TDA analysis was carried out in frontal mode for a better sensitivity of detection. The proof of concept was established using a model polyplex generated from the mixture of linear polylysine (DP 20) and DNA from salmon testes at nitrogen to phosphate (N/P) ratio of 12. Polyplex hydrodynamic radius was compared to the values obtained by dynamic light scattering measurements. TDA was found to give access to the weight-average hydrodynamic radius, while DLS basically gives an intensity-average (harmonic z-average) value. The method was next applied to the study of various polyplexes issued from polylysines of various DP (50, 100) and different topologies (dendrigraft polylysines of generation 2 and 3). This new methodology should greatly contribute to the physicochemical characterization of polyplexes used for g...
影响因子：
16.6
作者：
Anderson, DG;Lynn, DM;Langer, R
通讯作者：
Langer, R