Evolution and diversification of RNA silencing proteins in fungi
Evolution and diversification of RNA silencing proteins in fungi
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DOI：
10.1007/s00239-005-0257-2
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发表时间：
2006-07-01
影响因子：
3.9
通讯作者：
Mayama, Shigeyuki
中科院分区：
文献类型：
--
作者：
Nakayashiki, Hitoshi;Kadotani, Naoki;Mayama, Shigeyuki
对真菌Argonaute、Dicer和RNA依赖的RNA聚合酶样蛋白进行了全面的系统发育分析，以深入了解真菌进化过程中RNA沉默途径的多样化。包括子囊菌、担子菌和合子菌在内的多种真菌在基因组中具有多种RNA沉默成分，而部分子囊菌和担子菌真菌显然缺乏全部或大部分成分。真菌基因组中同源沉默蛋白的数量在不同物种之间差异很大，这表明RNA沉默途径在进化过程中随着生命周期复杂性的发展或对环境条件的响应而发生了显著的多样化。有趣的是，来自不同真菌分支的同源沉默蛋白通常比同源蛋白在真菌中聚集得更紧密，这表明复制事件发生在物种形成事件之前。因此，多种RNA沉默途径的起源似乎非常古老，可能发生在主要真菌谱系分化之前。
Comprehensive phylogenetic analyses of fungal Argonaute, Dicer, and RNA-dependent RNA polymerase-like proteins have been performed to gain insights into the diversification of RNA silencing pathways during the evolution of fungi. A wide range of fungi including ascomycetes, basidiomycetyes, and zygomycetes possesses multiple RNA silencing components in the genome, whereas a portion of ascomycete and basidiomycete fungi apparently lacks the whole or most of the components. The number of paralogous silencing proteins in the genome differs considerably among fungal species, suggesting that RNA silencing pathways have diversified significantly during evolution in parallel with developing the complexity of life cycle or in response to environmental conditions. Interestingly, orthologous silencing proteins from different fungal clades are often clustered more closely than paralogous proteins in a fungus, indicating that duplication events occurred before speciation events. Therefore, the origin of multiple RNA silencing pathways seems to be very ancient, likely having occurred prior to the divergence of the major fungal lineages.