Antioxidant activity of mycosporine-like amino acids isolated from three red macroalgae and one marine lichen
Antioxidant activity of mycosporine-like amino acids isolated from three red macroalgae and one marine lichen
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DOI：
10.1007/s10811-008-9345-1
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发表时间：
2009-04-01
影响因子：
3.3
通讯作者：
Herrera, E.
中科院分区：
文献类型：
--
作者：
de la Coba, F.;Aguilera, J.;Herrera, E.
为了确定从红藻紫菜中分离出的类菌孢素氨基酸 (MAA)、porphyra-334 加 shinorine (P-334 + SH)、从红藻中分离出的 asterina-330 加 plythine (AS-330 + PNE) 的纯化水提取物的潜在抗氧化能力，进行了多项标准体外测定。 角质菜石花菜素 (SH)，来自红藻 Ahnfeltiopsis devoniensis，而菌孢素-甘氨酸 (M-Gly)，则从海洋地衣 Lichina pygmaea 中分离出来。评估了水溶性自由基的清除能力（ABTS（+）脱色法）、脂质介质中的抗氧化活性（β-胡萝卜素/亚油酸漂白法）和超氧自由基的清除能力（连苯三酚自氧化测定）。在清除水溶性自由基方面，所有研究的 MAA 的抗氧化活性均呈剂量依赖性，并且随着介质的碱度（pH 6 至 8.5）而增加。 M-Gly 在所有 pH 测试中表现出最高的活性；在 pH 8.5 时，其 IC50 是 L-抗坏血酸 (L-ASC) 的 8 倍，其次是 AS-330 + PNE，而 P-334 + SH 和 SH 几乎没有清除水溶性自由基的活性。相对于维生素 E 和超氧自由基清除，AS-330 + PNE 显示出较高的 β-胡萝卜素氧化抑制活性，而 P-334 +SH 和 SH 的活性中等。根据这些结果，由于具有双重功能，MAA 在光保护方面的潜力可以被认为是很高的：（1）对太阳光谱的 UVB 和 UVA 区域进行高效的紫外线化学屏蔽，以及（2）它们的抗氧化能力。
Several standard in vitro assays were performed in order to determine the potential antioxidant capabilities of purified aqueous extracts of the mycosporine-like amino acids (MAAs), porphyra-334 plus shinorine (P-334 + SH), isolated from the red alga Porphyra rosengurttii, asterina-330 plus palythine (AS-330 + PNE), from the red alga Gelidium corneum, shinorine (SH), from the red alga Ahnfeltiopsis devoniensis, and mycosporine -glycine (M-Gly), isolated from the marine lichen Lichina pygmaea. The scavenging potential of hydrosoluble radicals (ABTS(+) decolorization method), the antioxidant activity in lipid medium (beta-carotene/ linoleate bleaching method) and the scavenging capacity of superoxide radicals (pyrogallol autooxidation assay) were evaluated. In terms of scavenging of hydrosoluble radicals, the antioxidant activity of all MAAs studied was dose-dependent and it increased with the alkalinity of the medium (pH 6 to 8.5). M-Gly presented the highest activity in all pH tested; at pH 8.5 its IC50 was 8-fold that of L-ascorbic acid (L-ASC) followed by AS-330 + PNE while P-334 + SH and SH showed scarce activity of scavenging of hydrosoluble free radicals. AS-330 + PNE showed high activity for inhibition of beta-carotene oxidation relative to vitamin E and superoxide radical scavenging whilst the activity of P-334 +SH and SH were moderate. According to these results, the potential of MAAs in photoprotection can be considered high due to a double function: (1) UV chemical screening with high efficiency for UVB and UVA regions of the solar spectrum, and (2) their antioxidant capacity.