MOLECULAR CHARACTERIZATION OF FISH NEUROLIN - A GROWTH-ASSOCIATED CELL-SURFACE PROTEIN AND MEMBER OF THE IMMUNOGLOBULIN SUPERFAMILY IN THE FISH RETINOTECTAL SYSTEM WITH SIMILARITIES TO CHICK PROTEIN DM-GRASP/SC-1/BEN
MOLECULAR CHARACTERIZATION OF FISH NEUROLIN - A GROWTH-ASSOCIATED CELL-SURFACE PROTEIN AND MEMBER OF THE IMMUNOGLOBULIN SUPERFAMILY IN THE FISH RETINOTECTAL SYSTEM WITH SIMILARITIES TO CHICK PROTEIN DM-GRASP/SC-1/BEN
复制标题
DOI：
10.1007/s002580050017
复制
发表时间：
1994-04-01
期刊：
影响因子：
2.9
通讯作者：
STUERMER, CAO
中科院分区：
文献类型：
--
作者：
LAESSING, U;GIORDANO, S;STUERMER, CAO
我们利用聚合酶链式反应分离了金鱼和斑马鱼神经素的编码基因，斑马鱼神经素是一种先前在金鱼视觉系统中发现的86 kDa细胞表面糖蛋白。序列分析表明，Neurlin属于免疫球蛋白超家族，与鸡细胞黏附分子DM-GRAP、SC-1、BEN有51%的相似性。用编码Neurlin C-末端的核探针进行Northern分析，在胚胎和成年金鱼中检测到两个3.7kb和3.3kb的mRNAs，并产生了几种针对免疫纯化的金鱼神经素的单抗和多克隆抗体，其中两种与斑马鱼蛋白发生交叉反应。这两种抗体在免疫印迹上都识别出一种与金鱼神经素分子量相同的斑马鱼蛋白。斑马鱼视网膜顶盖系统中这些抗体的免疫组织化学研究表明，年轻的神经节细胞上有标记，视网膜轴突的生长模式与金鱼相似。Neurlin与已知的细胞黏附分子的相似性，它在胚胎和成鱼发育中的视网膜神经节细胞和轴突上的表达，以及在金鱼视网膜轴突再生过程中的重新表达，表明Neurlin在鱼类中枢神经系统的轴突生长过程中起着重要的作用。
We have used the polymerase chain reaction to isolate cDNAs coding for goldfish and zebrafish neurolin, a previously identified 86 kDa cell surface glycoprotein in the goldfish visual system. Sequence analysis demonstrates that neurolin belongs to the immunoglobulin superfamily and is 51% similar to the chick cell adhesion molecule DM-GRASP, SC-1, BEN. Northern analysis with a riboprobe coding for the C-terminus of neurolin detected two mRNAs of 3.7 kb and 3.3 kb in both embryonic and adult goldfish.Several monoclonal and polyclonal antibodies were generated against immunopurified goldfish neurolin and two are shown to crossreact with zebrafish proteins. Both antibodies identify a zebrafish protein of the same molecular weight as goldfish neurolin on immunoblots. Immunohistochemical studies with these antibodies in the zebrafish retinotectal system demonstrate labeling on young ganglion cells and growing retinal axons in a pattern similar to that found in goldfish. The similarity of neurolin to a known cell adhesion molecule, its expression on developing retinal ganglion cells and axons in both embryos and adult fish, and its re-expression during retinal axon regeneration in the goldfish suggests that neurolin is important during axonal growth in the fish central nervous system.