Spatiotemporal Patterning of Zygotic Genome Activation in a Model Vertebrate Embryo
Spatiotemporal Patterning of Zygotic Genome Activation in a Model Vertebrate Embryo
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DOI：
10.1016/j.devcel.2019.05.036
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发表时间：
2019-06-17
影响因子：
11.8
通讯作者：
Good, Matthew C.
中科院分区：
文献类型：
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作者：
Chen, Hui;Einstein, Lily C.;Good, Matthew C.
早期胚胎发生的一个决定性特征是由母体控制向合子控制的转变。这种转变需要胚胎范围内的合子基因组激活（ZGA），但单个细胞之间ZGA的时空协调程度尚不清楚。多个相互关联的参数，包括经过的时间，完成的细胞分裂周期，和细胞大小可能会影响ZGA发作，然而，在脊椎动物胚胎发生ZGA的主要决定因素是有争议的。在这里，我们进行单细胞成像的大规模ZGA在整装非洲爪蟾胚胎。我们发现一个惊人的新的时空模式ZGA的发病密切相关的细胞大小，但不与经过的时间或细胞分裂的数量。此外，减小细胞大小诱导过早ZGA，呈剂量依赖性。我们的结论是，大规模ZGA在空间上并不均匀，它的发生是在单细胞水平上决定的，主要取决于细胞大小。我们的研究表明，ZGA发作的空间模式可能是胚胎系统的共同特征。
A defining feature of early embryogenesis is the transition from maternal to zygotic control. This transition requires embryo-wide zygotic genome activation (ZGA), but the extent of spatiotemporal coordination of ZGA between individual cells is unknown. Multiple interrelated parameters, including elapsed time, completed cycles of cell division, and cell size may impact ZGA onset; however, the principal determinant of ZGA during vertebrate embryogenesis is debated. Here, we perform single-cell imaging of large-scale ZGA in whole-mount Xenopus embryos. We find a striking new spatiotemporal pattern of ZGA whose onset tightly correlates with cell size but not with elapsed time or number of cell divisions. Further, reducing cell size induces premature ZGA, dose dependently. We conclude that large-scale ZGA is not spatially uniform and that its onset is determined at the single-cell level, primarily by cell size. Our study suggests that spatial patterns of ZGA onset may be a common feature of embryonic systems.