Transcription factor Spo0A switches the localization of the cell division protein FtsZ from a medial to a bipolar pattern in Bacillus subtilis
Transcription factor Spo0A switches the localization of the cell division protein FtsZ from a medial to a bipolar pattern in Bacillus subtilis
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DOI：
10.1101/gad.10.4.478
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发表时间：
1996-02-15
影响因子：
10.5
通讯作者：
Losick, R
中科院分区：
文献类型：
--
作者：
Levin, PA;Losick, R
革兰氏阳性细菌枯草芽孢杆菌进入孢子形成由两个转录因子Spo0A和sigma（H）控制，并且涉及分裂位点从中间位置到极性位置的转换。我们报告说，在发病的孢子形成，组装的细胞分裂蛋白FtsZ转移从中间细胞的潜在分裂位点附近的两极。FtsZ定位向双极模式的转换依赖于Spo0A。此外，在生长过程中合成活化形式的Spo0A人工激活FtsZ定位的开关，并导致极隔的形成。另一方面，对于在FtsZ组装位点的位置的开关，σ（H）因子是不确定的，尽管它是极隔形成所需的。我们的研究结果表明，在从生长到孢子形成的过渡过程中，Spo0A诱导基因的表达，抑制FtsZ组装在中间细胞的网站和激活网站在两极，而西格玛（H）诱导的基因所需的后续步骤在胞质分裂。
Entry into sporulation by the Gram-positive bacterium Bacillus subtilis is governed by two transcription factors, Spo0A and sigma(H), and involves a switch in the site of division from a medial to a polar location. We report that at the onset of sporulation, assembly of the cell division protein FtsZ shifts from midcell to potential division sites near both poles. The switch to a bipolar pattern of FtsZ localization is dependent on Spo0A. Additionally, synthesis of an activated form of Spo0A during growth artificially activates the switch in FtsZ localization and results in the formation of polar septa. The sigma(H) factor, on the other hand, is dispensable for the switch in the position of the FtsZ assembly site, although it is required for formation of the polar septum. Our results suggest that during the transition from growth to sporulation, Spo0A induces the expression of genes that suppress FtsZ assembly at the midcell site and activate sites at both poles, whereas sigma(H) induces genes required for a subsequent step in cytokinesis.