The use of bulk-tank milk ELISAs to assess the spatial distribution of Fasciola hepatica, Ostertagia ostertagi and Dictyocaulus viviparus in dairy cattle in Flanders (Belgium)
The use of bulk-tank milk ELISAs to assess the spatial distribution of Fasciola hepatica, Ostertagia ostertagi and Dictyocaulus viviparus in dairy cattle in Flanders (Belgium)
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DOI：
10.1016/j.vetpar.2009.07.006
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发表时间：
2009-10-28
影响因子：
2.6
通讯作者：
Charlier, J.
中科院分区：
文献类型：
--
作者：
Bennema, S.;Vercruysse, J.;Charlier, J.
肝片形吸虫、奥斯特线虫和胎生网尾线虫是温带地区牛的寄生蠕虫，分布广泛，对经济有重要影响。本文描述了肝片吸虫、O. ostertagi和D.产于弗兰德斯（比利时）的奶牛群中的胎生。从佛兰芒奶牛群（n = 7002）中随机选择1800个牛群，在社区水平上分层，以获得代表整个研究区域的样本。从每个牛群中，2006年秋季收集的散装牛奶样品用先前描述的抗体-ELISA进行分析，以确定寄生虫感染水平可能导致产量损失的牛群（F。hepatica和O. ostertagi）（定义为经济感染）或在过去的放牧季节（D.胎生）。经济性感染的群体流行。hepatica和O. ostertagi分别为37.3%（95%置信区间（CI）：35.1-39.7）和59.1%（95%CI：56.8-61.4）。D.胎生组为19.6%（95%CI：17.7-21.6）。在28.9%（CI 26.8-31.3）的牛群中，观察到所有三种蠕虫的感染水平较低。使用Moran's 1研究了（经济）感染聚集的存在，而使用空间扫描统计，空间关联的本地指标和核密度绘图研究了集群的位置和大小。在空间分布上，F. hepatica的空间分布呈轻度聚集性;观察到ostertagi。D.胎生感染在弗兰德斯分布均匀。了解高风险地区的位置可以提高认识，并可能是制定适合区域情况的控制措施的起点。(C)2009爱思唯尔有限公司版权所有。
Fasciola hepatica, Ostertagia ostertagi and Dictyocaulus viviparus are helminth parasites with a wide distribution and an important economic impact in cattle in temperate climates. This paper describes the spatial distribution of F hepatica, O. ostertagi and D. viviparus in dairy herds in Flanders (Belgium). One thousand eight hundred herds were selected at random from the Flemish dairy population (n = 7002), stratified on community level to obtain a sample representative for the entire study area. From each herd, a bulk milk sample collected in autumn 2006 was analysed with previously described antibody-ELISAs in order to identify herds where the parasite infection level is likely to cause production loss (F. hepatica and O. ostertagi) (defined as economic infections) or where patent infections have been present over the past grazing season (D. viviparus). The herd prevalence of economic infections with F. hepatica and O. ostertagi was 37.3% (95% Confidence Interval (Cl): 35.1-39.7) and 59.1% (95%CI: 56.8-61.4), respectively. The herd prevalence of D. viviparus was 19.6% (95%CI: 17.7-21.6). On 28.9% (CI 26.8-31.3) of the herds, low levels of infection were observed for all three of the helminths. The presence of clustering of (economic) infections was studied using Moran's 1, whereas the location and size of the clusters were studied using the spatial scan statistic, the Local Indicator of Spatial Association and Kernel density plotting. A marked clustering in the spatial distribution of F. hepatica and a mild clustering in the spatial distribution of O. ostertagi were observed. D. viviparus infections were spread evenly over Flanders. Knowledge of locations of high risk areas can lead to increased awareness and may be the start of the development of regionally adapted control measures. (C) 2009 Elsevier B.V. All rights reserved.