GABAergic neurons of the medial septum play a nodal role in facilitation of nociception-induced affect.
GABAergic neurons of the medial septum play a nodal role in facilitation of nociception-induced affect.
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DOI：
10.1038/srep15419
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发表时间：
2015-10-21
影响因子：
4.6
通讯作者：
Khanna S
中科院分区：
文献类型：
--
作者：
Ang ST;Lee AT;Foo FC;Ng L;Low CM;Khanna S
本研究通过操纵间隔内 GABA 能机制，探讨了内侧间隔区域 (MSDB) 对伤害性行为谱影响的功能细节。结果表明，福尔马林诱导的急性伤害感受不受中隔微量注射荷包牡丹碱（一种 GABAA 受体拮抗剂）或选择性损伤中隔 GABA 能神经元的影响。事实上，急性伤害性反应与 MSDB 中 GABA 能机制对感觉运动行为的调节和 θ 节律的产生无关。 GABA能损伤减弱了福尔马林诱导的前扣带皮层（ACC）中的非条件性细胞反应，并阻断了福尔马林诱导的条件性位置回避（F-CPA），以及短暂的足部电击条件性诱导的情境恐惧。然而，损伤对伤害性条件细胞反应的影响是可变的。有趣的是，在 F-CPA 任务中，损伤减弱了背侧海马区 CA1 实验环境的条件表征。总的来说，前面的内容表明 MSDB 是一个节点中心，其中 GABA 能神经元通过调节 ACC 中的细胞机制来介导伤害性情感动机，从而赋予伤害性刺激厌恶的价值。此外，结合对海马情境处理的调节影响，MSDB 可以将情感与情境结合起来，作为 F-CPA 任务中联想学习的一部分。
The present study explored the functional details of the influence of medial septal region (MSDB) on spectrum of nociceptive behaviours by manipulating intraseptal GABAergic mechanisms. Results showed that formalin-induced acute nociception was not affected by intraseptal microinjection of bicuculline, a GABAA receptor antagonist, or on selective lesion of septal GABAergic neurons. Indeed, the acute nociceptive responses were dissociated from the regulation of sensorimotor behaviour and generation of theta-rhythm by the GABAergic mechanisms in MSDB. The GABAergic lesion attenuated formalin-induced unconditioned cellular response in the anterior cingulate cortex (ACC) and blocked formalin-induced conditioned place avoidance (F-CPA), and as well as the contextual fear induced on conditioning with brief footshock. The effects of lesion on nociceptive-conditioned cellular responses were, however, variable. Interestingly, the lesion attenuated the conditioned representation of experimental context in dorsal hippocampus field CA1 in the F-CPA task. Collectively, the preceding suggests that the MSDB is a nodal centre wherein the GABAergic neurons mediate nociceptive affect-motivation by regulating cellular mechanisms in ACC that confer an aversive value to the noxious stimulus. Further, in conjunction with a modulatory influence on hippocampal contextual processing, MSDB may integrate affect with context as part of associative learning in the F-CPA task.