Genetic variability and population structure of the New World begomovirus Euphorbia yellow mosaic virus
Genetic variability and population structure of the New World begomovirus Euphorbia yellow mosaic virus
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DOI：
10.1099/jgv.0.000784
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发表时间：
2017-06-01
影响因子：
3.8
通讯作者：
Murilo Zerbini, F.
中科院分区：
文献类型：
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作者：
Mar, Talita Bernardon;Diniz Xavier, Cesar Augusto;Murilo Zerbini, F.
巴西贝戈莫病毒（属于双生病毒科贝戈莫病毒属的粉虱传播病毒）的出现可能是在引入烟粉虱 MEAM1 后通过非栽培植物的水平转移而发生的。异叶大戟（大戟科）多样性中心位于巴西和巴拉圭，是大豆和其他作物的入侵物种。关于巴西太子参可能感染 Begomovirus 的报道可以追溯到 20 世纪 50 年代。 2011 年，在巴西戈亚斯州收集的有症状植物中发现了大戟黄花叶病毒 (EuYMV)。在这里，我们评估了 2009 年至 2014 年在巴西九个州收集的样本中感染太子参的 begomoviruses 的遗传变异和种群结构。在 DNA-A 和 DNA-B 数据集中分别比较了总共 158 个和 57 个单倍型。通过对大样本区域的人口结构进行比较分析，我们能够区分两个亚种群。此外，主成分判别分析的应用允许根据采样位置区分六个亚群，并且与系统发育分析一致。一般来说，负选择在所有六个亚群中占主导地位。有趣的是，我们能够根据对提出的地理结构贡献最大的 23 个地点的信息重建系统发育，证明这些多态性拥有区分亚群的支持信息。这些位点被绘制在基因组中，并在氨基酸变化水平上进行比较，从地理结构的角度深入了解遗传漂变和选择如何有助于维持 Begomovirus 群体变异的模式。
The emergence of begomoviruses (whitefly-transmitted viruses classified in the genus Begomovirus, family Geminiviridae) in Brazil probably occurred by horizontal transfer from non-cultivated plants after the introduction of Bemisia tabaci MEAM1. The centre of diversity of Euphorbia heterophylla (Euphorbiaceae) is located in Brazil and Paraguay, where it is an invasive species in soybean and other crops. Reports of possible begomovirus infection of E. heterophylla in Brazil date back to the 1950s. In 2011, Euphorbia yellow mosaic virus (EuYMV) was described in symptomatic plants collected in the Brazilian state of Goias. Here we assess the genetic variability and population structure of begomoviruses infecting E. heterophylla in samples collected throughout nine Brazilian states from 2009 to 2014. A total of 158 and 57 haplotypes were compared in DNA-A and DNA-B datasets, respectively. Analysis comparing population structure in a large sampled area enabled us to differentiate two subpopulations. Further, the application of discriminant analysis of principal components allowed the differentiation of six subpopulations according to sampling locations and in agreement with phylogenetic analysis. In general, negative selection was predominant in all six subpopulations. Interestingly, we were able to reconstruct the phylogeny based on the information from the 23 sites that contributed most to the geographical structure proposed, demonstrating that these polymorphisms hold supporting information to discriminate between subpopulations. These sites were mapped in the genome and compared at the level of amino acid changes, providing insights into how genetic drift and selection contribute to maintain the patterns of begomovirus population variability from a geographical structuring point of view.