Evaluation of a mouse Y chromosome probe for assessing marrow transplantation.
Evaluation of a mouse Y chromosome probe for assessing marrow transplantation.
复制标题
用于评估骨髓移植的小鼠 Y 染色体探针的评估。
作者：
I. Hampson;E. Spooncer;T. Dexter
多拷贝小鼠 Y 染色体 DNA 探针 80Y/B (1) 已用于探测从男性和女性骨髓细胞中分离的基因组 DNA，这些细胞以已知的比例混合在一起。结果发现，在比例为 1 雄性：200 雌性骨髓细胞的混合物中，通过该方法可以容易地检测到雄性细胞。讨论了该技术用于评估骨髓移植的再增殖动力学的潜在用途。
The multicopy mouse Y chromosome DNA probe 80Y/B (1) has been used to probe genomic DNA isolated from male and female bone marrow cells, mixed together in known ratios. It was found that in a mixture with a ratio of 1 male:200 female marrow cells the male cells could readily be detected by this method. The potential use of this technique for assessing repopulation kinetics of marrow transplantation is discussed.