Conditioned medium from plateau-phase cells. Effect on growth of proliferative cells and on repair of potentially lethal radiation damage.
Conditioned medium from plateau-phase cells. Effect on growth of proliferative cells and on repair of potentially lethal radiation damage.
复制标题
来自平台期细胞的条件培养基。
作者：
I. A. Horowitz;H. Norwint;E. Hall
生长到平台期的体外细胞培养物可能比指数生长的细胞更适合作为体内肿瘤细胞生长的模型。从处于平台期的细胞中提取的条件培养基抑制相同或不同细胞系的对数期培养物的生长。试图证明这种抑制是由于热不稳定的扩散物质失败了。从平台期培养物中提取的条件培养基在增强x射线照射后潜在致死性损伤的修复中是有效的。与完全新鲜培养基相比，在条件培养基中辐射后培养6小时后，细胞存活率显著增加。
In vitro cell cultures grown into plateau phase may be more suitable than exponentially growing cells as a model for tumor cell growth in vivo. Conditioned medium extracted from cells in plateau phase inhibits growth of log-phase cultures of either the same or a different cell line. Attempts to demonstrate that this inhibition is due to a heat-labile diffusible substance have failed. Conditioned medium extracted from plateau-phase cultures was effective in enhancing repair of potentially lethal damage following x irradiation. Cell survival is significantly increased following a six-hour postirradiation culture in conditioned medium as opposed to complete fresh medium.