Structure of the bovine lactoferrin-encoding gene and its promoter.
Structure of the bovine lactoferrin-encoding gene and its promoter.
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牛乳铁蛋白编码基因及其启动子的结构。
DOI：
10.1016/0378-1119(94)90108-2
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发表时间：
1994
期刊：
影响因子：
3.5
通讯作者：
G. Hobom
中科院分区：
文献类型：
--
作者：
H. Seyfert;A. Tuckoricz;H. Interthal;D. Koczan;G. Hobom
乳铁蛋白 (Lf) 是一种三价铁离子 (Fe3+) 结合糖蛋白，主要存在于牛奶中，可能有助于预防乳腺微生物感染。基于牛 Lf 基因 (bLf) 完整 cDNA 的初步分离和测序，从五个重叠噬菌体 λ EMBL3 克隆的基因组文库中获得了完整基因。提供了该基因的详细限制性图谱和完整的外显子/内含子结构，以及近端外显子上游启动子的 1 kb 序列数据。编码序列分散在 17 个外显子上，横跨 34.5 kb 的基因组 DNA。虽然外显子大小与转铁蛋白基因家族 (Tf) 的其他成员相似，但某些内含子大小却截然不同。两个外显子大小的进化保守性及其对蛋白质分子各个结构域的贡献增加了证据，证明“外显子”是通过内部序列重复产生的。启动子序列缺乏人和小鼠Lf启动子中发现的一些转录增强子序列基序，这表明bLf表达相对较弱的潜在原因。
Lactoferrin (Lf), a ferric ion (Fe3+)-binding glycoprotein, is found most notably in milk, probably to mediate protection against microbial infection of the mammary gland. Based on an initial isolation and sequencing of a complete cDNA of the bovineLfgene (bLf), the complete gene was obtained from genomic libraries on five overlapping phage λ EMBL3 clones. A detailed restriction map and the complete exon/intron structure of the gene are presented, together with 1 kb of sequence data of the promoter upstream from the proximal exon. The coding sequence is dispersed over 17 exons spanning 34.5 kb of genomic DNA. While the exons are of similar size, as in other members of the transferrin gene family (Tf), some of the intron sizes are very different. Evolutionary conservation of both exon sizes and their contribution to the various domains of the protein molecule add to the evidence thatLforiginated via an internal sequence duplication. The promoter sequence lacks some of the sequence motifs for transcriptional enhancers found in the promoters of human and mouseLf, suggesting a potential reason for the relatively weak expression of bLf.