Adult chicken alpha-globin genes alpha A and alpha D: no anemic shock alpha-globin exists in domestic chickens.
Adult chicken alpha-globin genes alpha A and alpha D: no anemic shock alpha-globin exists in domestic chickens.
复制标题
成年鸡α珠蛋白基因αA和αD：家鸡中不存在贫血性休克α珠蛋白。
DOI：
10.1073/pnas.78.10.5998
复制
发表时间：
1981
影响因子：
11.1
通讯作者：
Engel,JD
中科院分区：
文献类型：
--
作者：
Dodgson,JB;McCune,KC;Rusling,DJ;Krust,A;Engel,JD
在鸡的α-珠蛋白连锁群中已鉴定出三个阿尔法型珠蛋白基因。没有其他阿尔法型基因被直接证明在这三个紧密连锁的基因中的任何一个的10千碱基对以内。这三个基因已经通过DNA序列分析得到了确凿的鉴定。位于链接组5‘端的基因是胚胎中的阿尔法型珠蛋白基因pi或pi’，中心基因对应于次要的成人α-珠蛋白αD，3‘末端的基因序列对应于先前描述的cDNA克隆序列，推测为贫血休克诱导的α-珠蛋白基因[Salser，W.A.，Cummings，I.，Liu，A.，Strommer，J.，Padayatty，J.&Clarke，P.(1979)《细胞和血红蛋白开关的分子调节》，[Salser，W.A.，Cummings，I.，Liu，A.，Strommer，J.，Padayatty，J.&Clarke，P.(1979)，《细胞和血红蛋白开关的分子调节》，编辑。Stamatoyannopoulos，G.&Nienheis，A.(Grune and Stratton，New York)，第621-643页；Richards，R.I.&Wells，J.R.E.(1980)J.Biol。化学。255,9306-9311]。几个组的工作人员已经分离到了α-S类基因克隆，但还没有人鉴定出与已发表的主要鸡α-珠蛋白的氨基酸序列相对应的cDNA克隆。我们鉴定了α-S类序列是从非贫血鸡网织红细胞中分离到的唯一丰富的类α-珠蛋白序列。因此，我们认为α-S类型的序列对应于真正的α-A-珠蛋白物种。
Three alpha-type globin genes have been identified in the alpha-globin linkage group of chickens. No other alpha-type genes hae been directly shown to be within 10 kilobase pairs of any of these three closely linked genes. These three genes have been conclusively identified by DNA sequence analysis. The gene at the 5' end of the linkage group is an embryonic alpha-type globin gene, pi or pi', and the central gene corresponds to the minor adult alpha- globin, alpha D. The 3'-terminal gene sequence corresponds to the sequence of cDNA clones previously described as "alpha S", presumed anemic shock-induced alpha-globin gene [Salser, W. A., Cummings, I., Liu, A., Strommer, J., Padayatty, J. & Clarke, P. (1979) in Cellular and Molecular Regulation of Hemoglobin Switching, eds. Stamatoyannopoulos, G. & Nienheis, A. (Grune and Stratton, New York), pp. 621-643; Richards, R. I. & Wells, J. R. E. (1980) J. Biol. Chem. 255, 9306-9311]. Several groups of workers have isolated alpha S-type cDNA clones but no one has identified a cDNA clone corresponding to the published amino acid sequence of the major chicken alpha-globin, alpha A. We have identified the alpha S-type sequence as the only abundant alpha-like globin sequence in cDNA clones made from reticulocyte mRNA isolated from nonanemic chickens. Therefore, we suggest that the alpha S-type sequence corresponds to the true alpha A-globin species.