Exploring the structural changes in nitrogen-fixing microorganisms of rhizosheath during the growth of Stipagrostis pennata in the desert.
Exploring the structural changes in nitrogen-fixing microorganisms of rhizosheath during the growth of Stipagrostis pennata in the desert.
复制标题
探讨沙漠针茅生长过程中根鞘固氮微生物的结构变化
DOI：
10.1042/bsr20201679
复制
发表时间：
2021-04-30
影响因子：
4
通讯作者：
Ge F
中科院分区：
文献类型：
--
作者：
Tian Y;Ma X;Li Y;Cheng C;An D;Ge F
摘要目的：荒漠系统中羽花针茅的根鞘是其生存的一种适应性特征。虽然微生物在促进根鞘氮素循环方面发挥着重要的生态作用，但固氮微生物群落的多样性和功能尚未完全了解。材料与方法：因此，本研究的目的是探讨根鞘固氮能力和固氮微生物在不同生长时期的丰度变化。pennata。通过测序对固氮菌nifH基因进行了测序，以确定固氮菌的结构和多样性。pennata在根鞘不同生长时期的生长情况。结果：共鉴定出1256个操作分类单位（OTU），分布于不同生长时期。每个样品中同时分布有5个OTU，果期微生物的丰度和多样性远高于其他时期。主要涉及4个门，其中变形菌门是所有类群中最丰富的。结论：总之，本研究对不同生长时期根鞘中固氮微生物的丰度和特征进行了研究。pennata。同时也阐明和表明了不同生长时期根鞘固氮微生物的结构。佩纳塔变了。
Abstract Purpose: Rhizosheath is an adaptive feature for the survival of Stipagrostis pennata in desert systems. Although microorganisms play important ecological roles in promoting the nitrogen cycle of rhizosheath, the diversity and function of nitrogen-fixing microorganism communities have not been fully understood. Materials and methods: Therefore, the aim of the present study is to explore the nitrogen fixation ability of rhizosheaths and the changes in abundance of nitrogen-fixing microorganisms at different growth periods of S. pennata. We sequenced the nifH gene through sequencing to identify the structure and diversity of nitrogen-fixing microorganisms of S. pennata at different growth periods of rhizosheaths. Results: A total of 1256 operational taxonomic units (OTUs) were identified by nifH sequence and distributed in different growth periods. There were five OTUs distributed in each sample at the same time, and the abundance and diversity of microorganisms in fruit period were much higher than those in other periods. Mainly four phyla were involved, among which Proteobacteria was the most abundant in all groups. Conclusions: In general, the present study investigated the abundance and characteristics of nitrogen-fixing microorganisms of rhizosheaths in different growth periods of S. pennata. It also may elucidate and indicate that the structure of nitrogen-fixing microorganisms of rhizosheaths in different growth periods of S. pennata had changed.