Fate of goblet cells in experimental colitis
Fate of goblet cells in experimental colitis
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DOI：
10.1023/a:1020147630032
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发表时间：
2002-10-01
影响因子：
3.1
通讯作者：
Dekker, J
中科院分区：
文献类型：
--
作者：
Makkink, MK;Schwerbrock, NMJ;Dekker, J
我们试图将 IL10(-/-) 小鼠慢性发炎大肠中杯状细胞形态的特征变化与杯状细胞基因表达的特定变化联系起来。在健康小鼠和IL10(-/-)小鼠中，大肠区域的杯状细胞形态和基因表达存在显着差异。粘蛋白 Muc2 是杯状细胞形态的主要决定因素，在大多数杯状细胞中表达，但仅在阿尔新蓝和高铁二胺染色呈阳性的细胞中表达。 TFF3 仅在一小部分杯状细胞中表达。 IL10(-/-)小鼠的发炎结肠仍然含有大量小而营养不良的杯状细胞，具有相似的组织化学染色以及Muc2和TFF3表达模式，这与经常报道的结肠炎中的“杯状细胞耗竭”相矛盾。从数量上看，IL10(-/-)小鼠中Muc2和TFF3水平保持相对稳定。远端 IL10(-/-) 结肠中的 Muc2 所含的硫酸盐残基明显少于对照，这可能会损害其保护特性。
We sought to correlate the characteristic changes in goblet cell morphology in the chronically inflamed large intestine of IL10(-/-) mice to specific changes in goblet cell gene expression. In healthy as well as IL10(-/-) mice, marked differences were found among the large intestinal regions in goblet cell morphology and gene expression. The mucin Muc2, which is a major determinant of goblet cell morphology, was expressed in most goblet cells, yet only in cells staining positive for both Alcian blue and high iron diamine. TFF3 was expressed in only a small subset of goblet cells. Inflamed colon of IL10(-/-) mice still contained high numbers of small, hypotrophic goblet cells with similar histochemical staining and Muc2 and TFF3 expression patterns, contradicting the often reported "goblet cell depletion" in colitis. Quantitatively, the Muc2 and TFF3 levels remained relatively stabile in IL10(-/-) mice. Muc2 in distal IL10(-/-) colon contained significantly less sulfate residues than in controls, which may compromise its protective properties.