Mammoth and Mastodon collagen sequences; survival and utility
Mammoth and Mastodon collagen sequences; survival and utility
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DOI：
10.1016/j.gca.2011.01.022
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发表时间：
2011-04-01
影响因子：
5
通讯作者：
Collins, M.
中科院分区：
文献类型：
--
作者：
Buckley, M.;Larkin, N.;Collins, M.
基于现有的非洲象基因组数据，并通过对鼻骨胶原蛋白消化的LC-MALDI-MS/MS和LC-ESI-MS/MS分析，提出了象目和哺乳目类群的近乎完整的胶原(I)序列。研究了两种已灭绝的猛犸象(巨型猛犸象和原始猛犸象)、已灭绝的美洲乳齿象(Mammut Americanum)、已灭绝的直角象(Eepas(Palaeoloxodon)antiquus)、现存的亚洲象(Eepas Max Us)和非洲象(Loxodonta Africana)的胶原蛋白序列覆盖率，并对两种电离技术进行了比较。对西伦顿森林床英国中更新世(Cromerian)沉积物中的两个疑似猛犸象化石进行了分析，以探讨多肽质谱学在化石鉴定中的潜在应用。尽管化石的年代不同，但仍获得了足够的多肽来鉴定它们是象形动物，并获得了足够的序列差异来区分象形和乳头象的胶原蛋白(I)。深入的LC-MS分析进一步未能识别出可用于可靠区分大象三个属(Eepas，Loxodonta和Mammutus)的多肽，这一观察结果与这些物种之间预测的氨基酸替换率一致。(C)2011爱思唯尔有限公司。保留所有权利。
Near-complete collagen (I) sequences are proposed for elephantid and mammutid taxa, based upon available African elephant genomic data and supported with LC-MALDI-MS/MS and LC-ESI-MS/MS analyses of collagen digests from proboscidean bone. Collagen sequence coverage was investigated from several specimens of two extinct mammoths (Mammuthus trogontherii and Mammuthus primigenius), the extinct American mastodon (Mammut americanum), the extinct straight-tusked elephant (Elephas (Palaeoloxodon) antiquus) and extant Asian (Elephas maximus) and African (Loxodonta africana) elephants and compared between the two ionization techniques used. Two suspected mammoth fossils from the British Middle Pleistocene (Cromerian) deposits of the West Runton Forest Bed were analysed to investigate the potential use of peptide mass spectrometry for fossil identification. Despite the age of the fossils, sufficient peptides were obtained to identify these as elephantid, and sufficient sequence variation to discriminate elephantid and mammutid collagen (I). In-depth LC-MS analyses further failed to identify a peptide that could be used to reliably distinguish between the three genera of elephantids (Elephas, Loxodonta and Mammuthus), an observation consistent with predicted amino acid substitution rates between these species. (C) 2011 Elsevier Ltd. All rights reserved.