THE UNIQUE ACTION FOR BONE METABOLISM OF 1-ALPHA,25-(OH)2D3-26,23-LACTONE
THE UNIQUE ACTION FOR BONE METABOLISM OF 1-ALPHA,25-(OH)2D3-26,23-LACTONE
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DOI：
10.1016/s0006-291x(85)80217-5
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
NORMAN, AW
中科院分区：
文献类型：
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作者：
KIYOKI, M;KURIHARA, N;NORMAN, AW
[23 (S),25 (R)]-1. α。在含有0.1%牛血清白蛋白的培养基中，25-二羟基维生素d3 -26,23-内酯([23(S),25（R)]-1. α,25-(OH) 2d3 -26,23-内酯）增加成骨细胞克隆MC3T3-E1碱性磷酸酶的剂量依赖性活性。在250 pg/ml时，最大激活酶活性为对照的1.6倍。代谢物也以剂量相关的方式增加胶原合成。[23 (S), 25日(R)) -1. alpha。，25-(OH) 2d3 -26,23-内酯对45Ca的动员作用虽小但明显降低，并阻断了1. α的吸收作用。在器官培养的小鼠颅骨中，25-二羟基维生素D3，但甲状旁腺激素不存在。显然,[23 (S), 25日(R)) -1. alpha。，25-(OH) 2d3 -26,23-内酯在体外刺激成骨细胞分化并抑制骨吸收。
[23 (S),25 (R)]-1.alpha.,25-Dihydroxyvitamin D3-26,23-lactone ([23(S),25(R)]-1.alpha.,25-(OH)2D3-26,23-lactone) increased dose-dependently alkaline phosphatase activity in osteoblastic cells, clone MC3T3-E1, in medium containing 0.1% bovine serum albumin. The maximal stimulated enzyme activity/mg protein was 1.6-fold over that of control cultures at 250 pg/ml. The metabolite also increased collagen synthesis in a dose-related fashion. [23(S),25(R)]-1.alpha.,25-(OH)2D3-26,23-lactone decreased slightly but significantly 45Ca mobilization, and blocked the resorptive action of 1.alpha.,25-dihydroxyvitamin D3, but not that of parathyroid hormone, in mouse calvaria in organ culture. Apparently, [23(S),25(R)]-1.alpha.,25-(OH)2D3-26,23-lactone stimulates the differentiation of osteoblasts and inhibits bone resorption in vitro.