The differentially-expressed proteome in Zn/Cd hyperaccumulator Arabis paniculata Franch. in response to Zn and Cd.
The differentially-expressed proteome in Zn/Cd hyperaccumulator Arabis paniculata Franch. in response to Zn and Cd.
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DOI：
10.1016/j.chemosphere.2010.10.030
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发表时间：
2011
期刊：
影响因子：
8.8
通讯作者：
Xiao-wen Zeng;R. Qiu;Rong-rong Ying;Ye-tao Tang;Lu Tang;Xiao-hang Fang
中科院分区：
文献类型：
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作者：
Xiao-wen Zeng;R. Qiu;Rong-rong Ying;Ye-tao Tang;Lu Tang;Xiao-hang Fang
Zn/Cd超积累植物阿拉伯芥（Arabis paniculata）对高浓度Zn和Cd具有耐受性。为了阐明锌镉胁迫下植物耐锌镉的分子基础，本研究采用蛋白质组学方法对A.圆锥花序。植物暴露于低和高锌或镉水平10天。采用双向电泳（2-DE）对各处理叶片蛋白质进行分离。结果表明，Zn处理后差异表达的蛋白质有19个，Cd处理后差异表达的蛋白质有18个。利用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF/TOF-MS）鉴定了19个锌响应蛋白中的17个和18个镉响应蛋白中的16个。所鉴定的蛋白质大多数参与能量代谢、外源性/抗氧化防御、细胞代谢、蛋白质代谢，提示了A.在一定程度上，莲子草对Zn和Cd的吸收途径具有相似性。但在Zn和Cd处理下，A.圆锥花序。这些结果表明A.莲子草对锌胁迫的抵抗主要是通过增强植物生长素的合成和蛋白质代谢等能量代谢来维持植物生长和纠正错误折叠的蛋白质。在Cd胁迫下，植物通过抗氧化/异生防御和细胞代谢来维持细胞内氧化还原平衡和金属转运。
The Zn/Cd hyperaccumulator Arabis paniculata is able to tolerate high level of Zn and Cd. To clarify the molecular basis of Zn and Cd tolerance, proteomic approaches were applied to identify proteins involved in Zn and Cd stress response in A. paniculata. Plants were exposed to both low and high Zn or Cd levels for 10 d. Proteins of leaves in each treatment were separated by 2-DE (two-dimensional electrophoresis). Nineteen differentially-expressed proteins upon Zn treatments and 18 proteins upon Cd treatments were observed. Seventeen out of 19 of Zn-responsive proteins and 16 out of 18 of Cd-responsive proteins were identified using MALDI-TOF/TOF-MS (matrix-assisted laser desorption/ionization time of flight mass spectrometry). The most of identified proteins were known to function in energy metabolism, xenobiotic/antioxidant defense, cellular metabolism, protein metabolism, suggesting the responses of A. paniculata to Zn and Cd share similar pathway to certain extend. However, the different metal defense was also revealed between Zn and Cd treatment in A. paniculata. These results indicated that A. paniculata against to Zn stress mainly by enhancement of energy metabolism including auxin biosynthesis and protein metabolism to maintain plant growth and correct misfolded proteins. In the case of Cd, plants adopted antioxidative/xenobiotic defense and cellular metabolism to keep cellular redox homeostasis and metal-transportation under Cd stress.