Detecting genomic aberrations by fluorescence in situ hybridization with quantum dots-labeled probes.
Detecting genomic aberrations by fluorescence in situ hybridization with quantum dots-labeled probes.
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DOI：
10.1166/jnn.2007.920
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发表时间：
2007-12
影响因子：
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通讯作者：
Zhengran Jiang;Ruiyun Li;N. Todd;S. Stass;F. Jiang
中科院分区：
文献类型：
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作者：
Zhengran Jiang;Ruiyun Li;N. Todd;S. Stass;F. Jiang
利用荧光原位杂交(FISH)技术检测肿瘤基因的基因组变化，将为癌症的诊断和治疗提供重要信息。为了有效和可靠地检测基因组的变化，我们将基因的基因组DNA直接连接到半导体量子点荧光团(QD)上，制备了新型的FISH探针。所生成的QD-基因组探针比有机染料标记的探针具有更高的光稳定性，并且在中期和间期细胞中都显示出高强度。直接标记探针允许以快速、简单的FISH程序检测基因组靶标，具有高灵敏度和特异性。此外，QD-基因组探针在肺癌标本中的应用可以可靠地显示癌细胞中的基因扩增。这些结果表明，QD-FISH探针可能在基础研究和临床应用中提供一种分析与癌症相关的基因组异常的有效方法。
Detection of genomic alterations of cancer genes by fluorescent in situ hybridization (FISH) will provide important information for cancer diagnosis and therapy. To effectively and reliably detect the genomic changes, we prepared novel FISH probes by directly conjugating genomic DNA of genes to semiconductor quantum dot fluorophores (QDs). The generated QD-genomic probes are substantially more photostable than the probes labeled with organic dye and show high intensity in both metaphase and interphase cell. The directly labeling probes allow detection of genomic targets in a fast and simple FISH procedure with high sensitivity and specificity. Furthermore, application of the QD-genomic probes in lung cancer specimens can reliably visualize gene amplification in cancer cells. These results suggest that the QD-FISH probes may offer an effective approach to analyze cancer-related genomic aberrations in basic research and clinical applications.