A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae
A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae
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DOI：
10.1016/s0378-1119(00)00083-4
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发表时间：
2000-04-04
期刊：
影响因子：
3.5
通讯作者：
Gallwitz, D
中科院分区：
文献类型：
--
作者：
De Antoni, A;Gallwitz, D
基因标签可以通过酵母中的同源重组来实现。Kan(T)标记基因在基因中断和表位标记实验中起着重要的作用。本文描述了包含标签-loxP-kanMX-loxP盒的新模块，它允许通过重复使用Kan(R)标记来标记不同的基因。(C)2000 Elsevier Science B.V.保留所有权利。
Gene tagging can be achieved by homologous recombination in yeast. The kan(t) marker gene plays an important role in PCR-mediated gene disruption and PCR-mediated epitope tagging experiments. In this paper, new modules containing a tag-loxP-kanMX-loxP cassette are described that allow tagging of different genes by using the kan(r) marker repeatedly. (C) 2000 Elsevier Science B.V. All rights reserved.