Miraflow, Soft Contact Lens Cleaner, activity against Acanthamoeba spp.
Miraflow, Soft Contact Lens Cleaner, activity against Acanthamoeba spp.
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Miraflow，软性隐形眼镜清洁剂，针对棘阿米巴属的活性。
DOI：
10.1097/icl.0000000000000112
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Ohashi Y
中科院分区：
文献类型：
--
作者：
Kobayashi T;Higuchi-Watanabe N;Shiraishi A ,Uno T;Ohashi Y
目的：研制含20%异丙醇的软性隐形眼镜清洁剂。本研究的目的是测定Miraflow对棘阿米巴滋养体和囊泡的活性。此外，测定Miraflow和多用途溶液（MPSs）联合使用对棘阿米巴囊肿的活性。方法：进行两项模拟使用研究。采用Spearman-Karber法测定单独暴露于Miraflow后，castellani菌株ATCC 50514和ATCC 50370或多食A. ATCC 30461的滋养体和包囊数量的对数减少的意义。为了检测Miraflow和MPS的联合抗棘阿米巴活性，还测定了暴露于Miraflow 1分钟后暴露于MPS (ReNu fresh) 4小时后囊肿数量的对数减少。结果：Miraflow对棘阿米巴滋养虫具有较强的抗虫活性。然而，1分钟处理对castellanii ATCC 50514的囊肿仅相对有效（减少1.1 log），而对其他2株的囊肿没有统计学意义上的减少。与Miraflow和MPS联合使用显示出对囊肿的活性，并且对a . castellanii ATCC 50514, a . castellanii ATCC 50370和a . polyhaga ATCC 30461的数量分别减少3.0,1.0或1.5对数。结论：暴露于Miraflow和MPS联合导致棘阿米巴囊肿的数量减少。
Objective:Miraflow is a cleaner for soft contact lens which contains 20% isopropyl alcohol. The purpose of this study was to determine the activity against Acanthamoeba trophozoites and cysts for Miraflow. In addition, to determine the activity of combined Miraflow and multipurpose solutions (MPSs) against Acanthamoeba cysts.Methods:Two simulated-use studies were conducted. The significance in the log reduction in the number of trophozoites and cysts of A. castellanii strains ATCC 50514 and ATCC 50370 or A. polyphaga ATCC 30461 after exposure to Miraflow alone was determined by the Spearman–Karber method. To examine the activity against Acanthamoeba of combined Miraflow and an MPS, the log reduction in the number of cysts after a 1-min exposure to Miraflow followed by a 4-hr exposure to MPS (ReNu fresh) was also determined.Results:Short-time exposure of 30 sec to Miraflow demonstrated activity against the Acanthamoeba trophozoites. However, a 1-min treatment was only relatively effective (1.1 log reduction) against the cysts of A. castellanii ATCC 50514, but no statistically significant reduction was observed for the cysts of the other 2 strains. The combined use with Miraflow and MPS demonstrated activity against the cysts, and a 3.0, 1.0, or 1.5 log reduction in the numbers was obtained for A. castellanii ATCC 50514, A. castellanii ATCC 50370, and A. polyphaga ATCC 30461, respectively.Conclusions:Exposure to combined Miraflow and MPS resulted in reductions in the number of Acanthamoeba cysts.