iTRAQ-Based Membrane Proteomics Reveals Plasma Membrane Proteins Change During HepaRG Cell Differentiation.
iTRAQ-Based Membrane Proteomics Reveals Plasma Membrane Proteins Change During HepaRG Cell Differentiation.
复制标题
DOI：
10.1021/acs.jproteome.6b00305
复制
发表时间：
2016-11
影响因子：
4.4
通讯作者：
Mingzhi Zhao;Feng Xu;Feilin Wu;Debin Yu;Na Su;Yao Zhang;Long Cheng;P. Xu
中科院分区：
文献类型：
--
作者：
Mingzhi Zhao;Feng Xu;Feilin Wu;Debin Yu;Na Su;Yao Zhang;Long Cheng;P. Xu
HepaRG细胞是一种稳定的双能肝祖细胞系，仅在分化后才显示肝细胞功能。然而，从未分化到分化状态的过渡，伴随着增殖迁移和分化的机制，仍然知之甚少，特别是那些蛋白质驻留在质膜。本研究采用iTRAQ标记的定量膜蛋白质组学方法，系统分析了HepaRG细胞分化过程中膜蛋白表达的变化。在849个定量的膜蛋白中，共鉴定出70个差异表达的膜蛋白。功能和疾病聚类分析证明，这些蛋白质中有11个参与增殖，迁移和分化。通过qRT-PCR和Western blot验证两个关键因素（MMP-14和OCLN）。MMP-14的阻断进一步证明了其在肿瘤细胞迁移过程中的重要功能。数据集已上传至ProteomeXchange，标识符为PXD 004752。
HepaRG cell, a stabilized bipotent liver progenitor cell line, exhibits hepatocyte functions only after differentiation. However, the mechanism of transition from nondifferentiated to differentiated states, accompanied by proliferation migration and differentiation, remains poorly understood, particularly those proteins residing in the plasma membrane. In this study, the membrane protein expression change of HepaRG cell during differentiation were systematically analyzed using an iTRAQ labeled quantitative membrane proteomics approach. A total of 70 membrane proteins were identified to be differentially expressed among 849 quantified membrane proteins. Function and disease clustering analysis proved that 11 of these proteins are involved in proliferation, migration, and differentiation. Two key factors (MMP-14 and OCLN) were validated by qRT-PCR and Western blot. Blockade of MMP-14 further demonstrated its important function during tumor cell migration. The data sets have been uploaded to ProteomeXchange with the identifier PXD004752.