Chemical modification of the small intestinal Na+/D-glucose cotransporter by amino group reagents. Evidence for a role of amino group(s) in the binding of the sugar.
Chemical modification of the small intestinal Na+/D-glucose cotransporter by amino group reagents. Evidence for a role of amino group(s) in the binding of the sugar.
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氨基试剂对小肠 Na /D-葡萄糖协同转运蛋白的化学修饰。
DOI：
10.1016/0005-2736(83)90039-1
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
G. Semenza
中科院分区：
文献类型：
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作者：
J. Weber;G. Semenza
一些氨基试剂抑制小肠Na+/d-葡萄糖协同转运蛋白，作为Na+依赖性葡萄糖转运的催化剂或作为Na+依赖性根皮苷配体进行测量。本文中研究的氨基与先前研究的氨基不同（Biber，J.，Weber，J.和Semenza，G.（1983）Biochim.生物化学生物Acta 728，429-437）：通过同时存在Na+加糖底物，这些被保护免于失活。因此，它们可能位于底物结合位点内。
Some amino group reagents inactivate the small-intestinal Na+/d-glucose cotransporter, as measured either as a catalyst of Na+-dependentd-glucose transport or as a Na+-dependent phlorizin ligand. The amino group(s) studied in this paper are not identical with those investigated previously (Biber, J., Weber, J. and Semenza, G. (1983) Biochim. Biochim. Acta 728, 429–437): these are protected from inactivation by the simultaneous presence of Na+plus sugar substrates. They are thus likely to be located within the substrate-binding site.