Clustering of plasma membrane-bound cytochrome b5 reductase within 'lipid raft' microdomains of the neuronal plasma membrane
Clustering of plasma membrane-bound cytochrome b5 reductase within 'lipid raft' microdomains of the neuronal plasma membrane
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DOI：
10.1016/j.mcn.2008.08.013
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发表时间：
2009-01-05
影响因子：
3.5
通讯作者：
Gutierrez-Merino, Carlos
中科院分区：
文献类型：
--
作者：
Samhan-Arias, Alejandro K.;Angel Garcia-Bereguiain, Miguel;Gutierrez-Merino, Carlos
质膜氧化还原中心在神经元防御氧化应激和生存中发挥着重要作用。在培养的小脑颗粒神经元 (CGN) 中，大量黄素蛋白与质膜相关，并且 CGN 绿色/橙色自发荧光的强度与细胞色素 b(5) 还原酶的表达水平相关。通过荧光共振能量转移对 CGN 质膜中细胞色素 b(5) 还原酶的区域化指出了细胞色素 b(5) 还原酶与“脂筏”标记物霍乱毒素 B 和 Caveolin-2 之间的紧密接近性。这项研究揭示了膜结合细胞色素 b(5) 还原酶在神经元胞体的神经元间接触位点大量富集，并与 CGN 质膜的“脂筏”相关。我们还表明，细胞色素 b(5) 还原酶对 NADH 氧化酶活性和纯化的大鼠脑突触质膜的红移黄素荧光有很大贡献。总之，膜结合细胞色素 Lis 还原酶形成与神经元质膜相关的氧化还原中心大网。 (C) 2008 Elsevier Inc. 保留所有权利。
Plasma membrane redox centres play a major role in neuronal defence against oxidative stress and survival. in cerebellar granule neurons in culture (CGN) a large pool of the flavoproteins are associated with the plasma membrane, and the intensity of CGN green/orange autofluorescence Correlated with the levels of expression of cytochrome b(5) reductase. Regionalization of cytochrome b(5) reductase in the plasma membrane of CGN by fluorescence resonance energy transfer points Out the close proximity between cytochrome b(5) reductase and the 'lipid raft' markers cholera toxin B and caveolin-2. This study unravels that membrane-bound cytochrome b(5) reductase is largely enriched at interneuronal contact sites in the neuronal soma and associated with 'lipid rafts' of the CGN plasma membrane. We also Show that cytochrome b(5) reductase makes a large contribution to the NADH oxidase activity and to the red-shifted flavine fluorescence of purified rat brain synaptic plasma membranes. In conclusion, membrane-bound cytochrome Lis reductase forms a large mesh of redox centres associated with the neuronal plasma membrane. (C) 2008 Elsevier Inc. All rights reserved.