Bioluminescent bioreporter integrated circuits (BBICs)
Bioluminescent bioreporter integrated circuits (BBICs)
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生物发光生物报告集成电路 (BBIC)
DOI：
10.1117/12.320171
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
G. Jellison
中科院分区：
文献类型：
--
作者：
M. Simpson;G. Sayler;D. Nivens;S. Ripp;M. Paulus;G. Jellison
作为集成电路（IC）工业的主力，CMOS的能力已经远远超出了最初的应用。从开关电容滤波器到微波电路模块，从通用运算放大器到核物理实验中的亚纳秒级模拟定时电路，各种模拟电路都已在CMOS中实现。该技术还利用诸如有源像素传感器和其他光电检测设备的设备进入了单片传感器的不断增长的领域。虽然许多用于MEMS制造的工艺与CMOS IC工艺不兼容，但将传感器材料沉积到先前制造的CMOS电路上可以创建非常有用的传感器类别。在这项工作中，我们报告了一个化学传感器组成的生物发光生物报告（基因工程菌）沉积到一个微光度计在一个标准的CMOS IC工艺制造。用于这项工作的生物报告器在暴露于甲苯时发出490 nm的光。通过微光度计检测这种发光，给出甲苯浓度的指示。已经报道了对爆炸物、汞和其他有机化学品和重金属敏感的其他生物发光生物报告物。这些可以与本文报道的微光度计结合（单独或组合）以形成各种化学传感器。
As the workhorse of the integrated circuit (IC) industry, the capabilities of CMOS have been expanded well beyond the original applications. The full spectrum of analog circuits from switched-capacitor filters to microwave circuit blocks, and from general-purpose operational amplifiers to sub- nanosecond analog timing circuits for nuclear physics experiments have been implemented in CMOS. This technology has also made in-roads into the growing area of monolithic sensors with devices such as active-pixel sensors and other electro-optical detection devices. While many of the processes used for MEMS fabrication are not compatible with the CMOS IC process, depositing a sensor material onto a previously fabricated CMOS circuit can create a very useful category of sensors. In this work we report a chemical sensor composed of bioluminescent bioreporters (genetically engineered bacteria) deposited onto a micro-luminometer fabricated in a standard CMOS IC process. The bioreporter used for this work emitted 490-nm light when exposed to toluene. This luminescence was detected by the micro- luminometer giving an indication of the concentration of toluene. Other bioluminescent bioreporters sensitive to explosives, mercury, and other organic chemicals and heavy metals have been reported. These could be incorporated (individually or in combination) with the micro-luminometer reported here to form a variety of chemical sensors.