Gene expression profiles and structural/functional features of the peri-implantation endometrium in natural and gonadotropin-stimulated cycles
Gene expression profiles and structural/functional features of the peri-implantation endometrium in natural and gonadotropin-stimulated cycles
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DOI：
10.1210/jc.2004-0605
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发表时间：
2004-11-01
影响因子：
5.8
通讯作者：
Oehninger, S
中科院分区：
文献类型：
--
作者：
Mirkin, S;Nikas, G;Oehninger, S
据推测，在体外受精治疗期间进行的控制性超排卵(COH)可能会对胚胎着床产生负面影响。这项前瞻性随机研究的目的是研究人子宫内膜在促性腺激素刺激的COH周期植入窗口期间的基因表达谱，并与时间上匹配的自然周期(D 21)进行比较。用高密度寡核苷酸微阵列进行分析。此外，还对子宫内膜的其他结构和功能特征进行了研究。结果证实，与自然周期相比，COH周期描述了针脚的外观、组织学特征和类固醇受体下调的进步。这些变化与基因表达的显著差异有关(18个基因/表达序列标签和-1.55-+3.40倍的变化)。其次，当比较使用GnRH激动剂和GnRH拮抗剂的周期时，基因表达发生了显著变化(13个基因/表达序列标签和+1.42-到+2.10倍的变化)。这是首次尝试阐明COH周期中子宫内膜的基因表达谱。使用最先进的方案在COH周期中观察到的基因表达差异可能不会对胚胎植入产生重大的功能影响。
It has been speculated that controlled ovarian hyperstimulation (COH), as performed during in vitro fertilization therapy, may negatively affect embryo implantation. The objective of this prospective and randomized study was to investigate gene expression profiles of the human endometrium during the window of implantation of gonadotropin-stimulated COH cycles compared with temporally matched natural cycles (d 21). Analysis was performed with high-density oligonucleotide microarrays. In addition, other structural and functional features of the endometrium were investigated. Results corroborated that COH cycles depicted advancement of pinopodes appearance, histological features, and steroid receptor down-regulation when compared with natural cycles. These changes were associated with significant, albeit small, variations in gene expression (18 genes/expressed sequence tags and -1.55- to +3.40-fold changes). Second, there were significant changes in gene expression when comparing cycles using a GnRH agonist vs. a GnRH antagonist (13 genes/expressed sequence tags and +1.42- to +2.10-fold changes). This is the first attempt to elucidate gene expression profiles of the endometrium during COH cycles. The observed differences in gene expression in COH cycles using state-of-the-art protocols may not have a major functional impact on embryo implantation.