F4-80, A MONOCLONAL-ANTIBODY DIRECTED SPECIFICALLY AGAINST THE MOUSE MACROPHAGE
F4-80, A MONOCLONAL-ANTIBODY DIRECTED SPECIFICALLY AGAINST THE MOUSE MACROPHAGE
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DOI：
10.1002/eji.1830111013
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发表时间：
1981-01-01
影响因子：
5.4
通讯作者：
GORDON, S
中科院分区：
文献类型：
--
作者：
AUSTYN, JM;GORDON, S
一种分泌巨噬细胞（M.PHI.）用培养的巯基乙酸诱导的小鼠腹膜M. PHI超免疫大鼠脾细胞，融合产生特异性单克隆抗体F4/80。小鼠骨髓瘤NS 1通过放射免疫间接结合试验、放射自显影或荧光激活细胞分选仪分析检测抗体与原代细胞和细胞系的结合。F4/80结合小鼠M.PHI.来自腹膜腔或其他来源的血单核细胞、M.PHI.来源于培养物中的骨髓前体和M.如细胞系，但不与其他细胞，包括多形核白细胞、淋巴细胞或成纤维细胞。F4/80不与M. PHI结合。通过Fc受体，无细胞毒性，属于大鼠IgG 2b亚类。由于F4/80与所有的M.PHI.通过粘附、形态学和免疫吞噬作用来定义，它提供了一种新的标记来定义M. PHI。鼠标中。发现抗原F4/80的表达存在很大差异，这取决于腹膜内刺激、培养时间和成熟阶段。免疫沉淀实验证明抗原F4/80是由M. PHI合成的MW 160，000组分的一部分。并且至少部分暴露在细胞表面上。
A hybridoma clone which secretes a macrophage (M.PHI.)-specific monoclonal antibody, F4/80, was produced by fusing spleen cells from a rat hyperimmunized with cultured thioglycollate-induced mouse peritoneal M.PHI. with a mouse myeloma, NS1. Binding of antibody to primary cells and cell lines was detected by radioimmune indirect binding assay, autoradiography or fluorescence-activated cell sorter analysis. F4/80 binds to mouse M.PHI. from the peritoneal cavity or other sources, blood monocytes, M.PHI. derived from bone marrow precursors in culture and M.PHI.-like cell lines, but not to other cells, including polymorphonuclear leukocytes, lymphocytes or fibroblasts. F4/80 does not bind to M.PHI. via Fc receptors, is not cytotoxic and is of the rat IgG2b subclass. Since F4/80 binds to all M.PHI. defined by adherence, morphology and immune phagocytosis, it provides a new marker to define the M.PHI. in the mouse. Large differences in expression of antigen F4/80 were found, depending on i.p. stimulation, time in culture and stage of maturation. Immunoprecipitation experiments demonstrated the the antigen F4/80 is part of a component of MW 160,000 which is synthesized by the M.PHI. and, at least in part, exposed on the cell surface.