Independent wheat B and G genome origins in outcrossing Aegilops progenitor haplotypes
Independent wheat B and G genome origins in outcrossing Aegilops progenitor haplotypes
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DOI：
10.1093/molbev/msl151
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发表时间：
2007-01-01
影响因子：
10.7
通讯作者：
Salamini, F.
中科院分区：
文献类型：
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作者：
Kilian, B.;Ozkan, H.;Salamini, F.
现代小麦的起源涉及相关基因组之间的同种异体倍化。为了确定Aegilops speltoides是否是AABB和AAGG四倍体中B和G基因组的供体，我们采用了三层方法。利用70个扩增片段长度多态性（AFLP）位点，对来自自然生境的480个小麦品系的分子多样性进行了采样，其中包括所有S基因组的Aegilops，即小麦B和G基因组的推定祖先。在375个AFLP位点上与二倍体、四倍体和11个非四体小麦品系进行了比较。B基因组特异性标记允许将B基因组的起源固定在A. speltoides的S染色体上，而排除了其他谱系。异交性影响了其分子多样性，并影响了B和G基因组起源的推断。与70个小麦品系（0.73 Mb序列）相似的核和叶绿体ACC1、G6PDH、GPT、PGK1、Q、VRN1和ndhF单倍型显示，多倍体小麦的B和G基因组都是小麦单倍型多样性的独特样本。它们在独立起源时被AABB的Triticum dicoccoides和AAGG的Triticum araraticum所隔离。
The origin of modern wheats involved alloploidization among related genomes. To determine if Aegilops speltoides was the donor of the B and G genomes in AABB and AAGG tetraploids, we used a 3-tiered approach. Using 70 amplified fragment length polymorphism (AFLP) loci, we sampled molecular diversity among 480 wheat lines from their natural habitats encompassing all S genome Aegilops, the putative progenitors of wheat B and G genomes. Fifty-nine Aegilops representatives for S genome diversity were compared at 375 AFLP loci with diploid, tetraploid, and 11 nulli-tetrasomic Triticum aestivum wheat lines. B genome-specific markers allowed pinning the origin of the B genome to S chromosomes of A. speltoides, while excluding other lineages. The outbreeding nature of A. speltoides influences its molecular diversity and bears upon inferences of B and G genome origins. Haplotypes at nuclear and chloroplast loci ACC1, G6PDH, GPT, PGK1, Q, VRN1, and ndhF for similar to 70 Aegilops and Triticum lines (0.73 Mb sequenced) reveal both B and G genomes of polyploid wheats as unique samples of A. speltoides haplotype diversity. These have been sequestered by the AABB Triticum dicoccoides and AAGG Triticum araraticum lineages during their independent origins.