Antioxidant activities of compounds isolated from Dalbergia odorifera T. Chen and their inhibition effects on the decrease of glutathione level of rat lens induced by UV irradiation
Antioxidant activities of compounds isolated from Dalbergia odorifera T. Chen and their inhibition effects on the decrease of glutathione level of rat lens induced by UV irradiation
复制标题
降香黄檀化合物的抗氧化活性及其对紫外线照射引起的大鼠晶状体谷胱甘肽水平下降的抑制作用
DOI：
10.1016/j.foodchem.2006.10.081
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发表时间：
2007-01-01
期刊：
影响因子：
8.8
通讯作者：
Yang, Ming
中科院分区：
文献类型：
--
作者：
Yu, Xiaolin;Wang, Wen;Yang, Ming
从降香黄檀（Dalbergia odorifera T.）通过化学和光谱方法鉴定为2 '-O-甲基异甘草素（1）、丁香烯（2）、5 '-甲氧基前庭醇（3）和芒柄花素（4）。采用氧化稳定性仪研究了它们在猪油中的抗氧化活性;采用大鼠透镜紫外线损伤模型研究了它们对紫外线照射引起的大鼠透镜谷胱甘肽水平下降的抑制作用;并测定了它们对人神经母细胞瘤细胞SH-SY 5 Y的体外细胞毒性。结果表明，4种化合物均具有明显的抗氧化作用，但对丁羟甲苯和α-生育酚无协同抗氧化作用。当FeCl 3（4.0 μ M）的加入，四种化合物的抗氧化活性显着下降。化合物1、2和4对紫外线照射引起的大鼠透镜谷胱甘肽水平下降的抑制作用与α-生育酚相当。所有四种化合物在体外对SH-SY 5 Y细胞系进行评价时均显示出细胞毒性活性。(C)2007爱思唯尔有限公司保留所有权利。
Four compounds were isolated from the root of Dalbergia odorifera T. Chen and identified by chemical and spectroscopic methods as 2 '-O-methyl-isoliquiritigenin (1), odoriflavene (2), 5 '-methoxy-vestitol (3) and formononetin (4). Their antioxidant activities were investigated in lard by the oxidative stability instrument; their inhibitory effects on the decrease of glutathione level of rat lens induced by UV irradiation were studied using a rat lens UV-damage model, and their in vitro cytotoxicity was measured on human SH-SY5Y neuroblastoma cells. Results indicated that all four compounds had obvious antioxidant effects, while none of them showed any synergistic effects on butylated hydroxytoluene or alpha-tocopherol. When FeCl3 (4.0 mu M) was added, the antioxidant activities of the four compounds markedly decreased. The inhibitory effects of compounds 1, 2 and 4 on the decrease of glutathione level of rat lens induced by UV irradiation were comparable to that of alpha-tocopherol. All four compounds showed cytotoxic activity when evaluated against a SH-SY5Y cell line in vitro. (C) 2007 Elsevier Ltd. All rights reserved.