The cell morphogenesis gene SPIRRIG in Arabidopsis encodes a WD/BEACH domain protein
The cell morphogenesis gene SPIRRIG in Arabidopsis encodes a WD/BEACH domain protein
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DOI：
10.1111/j.1365-313x.2009.03900.x
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发表时间：
2009-08-01
期刊：
影响因子：
7.2
通讯作者：
Huelskamp, Martin
中科院分区：
文献类型：
--
作者：
Saedler, Rainer;Jakoby, Marc;Huelskamp, Martin
WD40/BEACH结构域蛋白参与盘形骨菌和果蝇的膜转运和膜组成事件。在本文中，我们发现拟南芥SPIRRIG （SPI）基因编码一个WD40/BEACH结构域蛋白。细胞分析显示，根毛上的碎片液泡与相应的盘基骨柱突变体中发现的液泡相似，表明存在相关的细胞功能。表型分析显示，spi突变体具有肌动蛋白聚合调控ARP2/3通路突变体的所有表型特征，包括毛状体扭曲、表皮铺路细胞少裂、各器官表皮细胞断连以及根毛变短。这种完全的表型重叠表明，WD40/BEACH结构域蛋白和肌动蛋白调节ARP2/3通路参与了类似的生长过程。
WD40/BEACH domain proteins have been implicated in membrane trafficking and membrane composition events in Dictyostelium and Drosophila. In this paper, we show that the Arabidopsis SPIRRIG (SPI) gene encodes a WD40/BEACH domain protein. The cellular analysis revealed fragmented vacuoles in root hairs similar to those found in the corresponding Dictyostelium mutants, suggesting a related cellular function. The phenotypic analysis revealed that spi mutants share all phenotypic aspects of mutants in the actin polymerization-regulating ARP2/3 pathway, including distorted trichomes, less lobing of epidermal pavement cells, disconnected epidermal cells on various organs, and shorter root hairs. This complete phenotypic overlap suggests that this WD40/BEACH domain protein and the actin-regulating ARP2/3 pathway are involved in similar growth processes.